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| Registros recuperados : 4 | |
| 2. |  | GONÇALVES, G. S.; PEZZATO, L. E.; BARROS, M. M.; ROCHA, D. F.; KLEEMAN, G. K.; SANTA ROSA, M. J. Energia e nutrientes digestíveis de alimentos para a tilápia do Nilo. Boletim do Instituto de Pesca, São Paulo, SP, v. 35, n. 2, p. 201-213, abr./jun. 2009.| Biblioteca(s): Epagri-Sede. |
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| 3. | | ROCHA, D. F. O.; CUNHA, C. M. S.; BELAZ, K. R. A.; SANTOS, F. N.; HINZ, R. H.; PEREIRA, A.; VISCONTI, A.; WICKERT, E.; ANDRADE, L. M.; NASCIMENTO, C. A. O.; EBERLIN, M. N. MALDI-TOF MS Fingerprinting as a Convenient Tool to Select Small Proteins Related to Pathogen Adaptation and Virulence in Panama Disease. In: WORLD CHEMISTRY CONGRESS, 46., 2017, São Paulo. Abstracts. São Paulo: IUPAC, 2017.| Biblioteca(s): Epagri-Sede. |
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| 4. | | ROCHA, D. F. O.; CUNHA, C. M. S.; BELAZ, K. R. A.; SANTOS, F. N.; HINZ, R. H.; PEREIRA, A.; WICKERT, E.; ANDRADE, L. M.; NASCIMENTO, C. A. O.; VISCONTI, A.; EBERLIM, M. N. Lipid and protein fingerprinting for Fusarium oxysporum f. sp. cubense strain-level classification. Analytical and Bioanalytical Chemistry, Germany, v. 409, n. 29, p. 6803-6812, 2017.| Biblioteca(s): Epagri-Sede. |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
16/11/2017 |
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Data da última atualização: |
16/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
ROCHA, D. F. O.; CUNHA, C. M. S.; BELAZ, K. R. A.; SANTOS, F. N.; HINZ, R. H.; PEREIRA, A.; WICKERT, E.; ANDRADE, L. M.; NASCIMENTO, C. A. O.; VISCONTI, A.; EBERLIM, M. N. |
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Título: |
Lipid and protein fingerprinting for Fusarium oxysporum f. sp. cubense strain-level classification. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Analytical and Bioanalytical Chemistry, Germany, v. 409, n. 29, p. 6803-6812, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Banana is one of the most popular fruits in the world but has been substantially impaired by Panama disease in the last years. Fusarium oxysporum f. sp. cubense (Foc) is the causal agent and colonizes banana cultivars from many subgroups with different aggressiveness levels, often leading to plant death while compromising new crops in infested areas. This study has evaluated the ability of MALDI-MS protein and lipid fingerprinting to provide intraspecies classification of Foc isolates and to screen biomolecules related to host-pathogen relationship. The MS data, when inspected via partial least square discriminant analysis (PLS-DA), distinguished the isolates by aggressiveness as well as by specific location and host. Although both lipids and proteins show discriminating tendencies, these differences were more clearly perceived via the protein profiles. Considering that Cavendish cultivar is the more resistant option to endure Foc presence in the field, the lipids and proteins related to this subgroup might have an important role in pathogen adaptation. This study reports a new application of MALDI-MS for the analysis of a banana pathogen with intraspecies classification ability. |
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Palavras-Chave: |
LMW proteins; MALDI-MS; Microbial classification; Multivariate statistical analysis; Phospholipids; Phytopathogen. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02140naa a2200313 a 4500
001 1126868
005 2017-11-16
008 2017 bl uuuu u00u1 u #d
100 1 $aROCHA, D. F. O.
245 $aLipid and protein fingerprinting for Fusarium oxysporum f. sp. cubense strain-level classification.$h[electronic resource]
260 $c2017
520 $aBanana is one of the most popular fruits in the world but has been substantially impaired by Panama disease in the last years. Fusarium oxysporum f. sp. cubense (Foc) is the causal agent and colonizes banana cultivars from many subgroups with different aggressiveness levels, often leading to plant death while compromising new crops in infested areas. This study has evaluated the ability of MALDI-MS protein and lipid fingerprinting to provide intraspecies classification of Foc isolates and to screen biomolecules related to host-pathogen relationship. The MS data, when inspected via partial least square discriminant analysis (PLS-DA), distinguished the isolates by aggressiveness as well as by specific location and host. Although both lipids and proteins show discriminating tendencies, these differences were more clearly perceived via the protein profiles. Considering that Cavendish cultivar is the more resistant option to endure Foc presence in the field, the lipids and proteins related to this subgroup might have an important role in pathogen adaptation. This study reports a new application of MALDI-MS for the analysis of a banana pathogen with intraspecies classification ability.
653 $aLMW proteins
653 $aMALDI-MS
653 $aMicrobial classification
653 $aMultivariate statistical analysis
653 $aPhospholipids
653 $aPhytopathogen
700 1 $aCUNHA, C. M. S.
700 1 $aBELAZ, K. R. A.
700 1 $aSANTOS, F. N.
700 1 $aHINZ, R. H.
700 1 $aPEREIRA, A.
700 1 $aWICKERT, E.
700 1 $aANDRADE, L. M.
700 1 $aNASCIMENTO, C. A. O.
700 1 $aVISCONTI, A.
700 1 $aEBERLIM, M. N.
773 $tAnalytical and Bioanalytical Chemistry, Germany$gv. 409, n. 29, p. 6803-6812, 2017.
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