02140naa a2200313 a 450000100080000000500110000800800410001910000200006024501270008026000090020752012030021665300170141965300130143665300290144965300380147865300180151665300180153470000200155270000200157270000180159270000160161070000160162670000160164270000190165870000250167770000170170270000190171977300880173811268682017-11-16       2017    bl uuuu     u00u1 u    #d1 aROCHA, D. F. O.  aLipid and protein fingerprinting for Fusarium oxysporum f. sp. cubense strain-level classification.h[electronic resource]  c2017  aBanana is one of the most popular fruits in the world but has been substantially impaired by Panama disease in the last years. Fusarium oxysporum f. sp. cubense (Foc) is the causal agent and colonizes banana cultivars from many subgroups with different aggressiveness levels, often leading to plant death while compromising new crops in infested areas. This study has evaluated the ability of MALDI-MS protein and lipid fingerprinting to provide intraspecies classification of Foc isolates and to screen biomolecules related to host-pathogen relationship. The MS data, when inspected via partial least square discriminant analysis (PLS-DA), distinguished the isolates by aggressiveness as well as by specific location and host. Although both lipids and proteins show discriminating tendencies, these differences were more clearly perceived via the protein profiles. Considering that Cavendish cultivar is the more resistant option to endure Foc presence in the field, the lipids and proteins related to this subgroup might have an important role in pathogen adaptation. This study reports a new application of MALDI-MS for the analysis of a banana pathogen with intraspecies classification ability.  aLMW proteins  aMALDI-MS  aMicrobial classification  aMultivariate statistical analysis  aPhospholipids  aPhytopathogen1 aCUNHA, C. M. S.1 aBELAZ, K. R. A.1 aSANTOS, F. N.1 aHINZ, R. H.1 aPEREIRA, A.1 aWICKERT, E.1 aANDRADE, L. M.1 aNASCIMENTO, C. A. O.1 aVISCONTI, A.1 aEBERLIM, M. N.  tAnalytical and Bioanalytical Chemistry, Germanygv. 409, n. 29, p. 6803-6812, 2017.