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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
16/04/2013 |
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Data da última atualização: |
16/04/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NASCIMENTO, D. D.; ANDRADE, A.; LABATE, C. A.; VENEZIANO, L. M. T.; GUTMANIS, G.; BERTOLO, A. L. ; BRAGATTO, J |
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Afiliação: |
Epagri |
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Título: |
Modulating MIOX2 expression in Nicotiana tabacum and impacts on genes involved in cell wall biosynthesis. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Bioenergia em Revista, Piracicaba, SP, v. 2, n. 1, p. 60-82, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Cell walls are essential structures for plant development and growth. Apart from its biological functions, the polysaccharides that make cell walls (cellulose, hemicellulose and pectins) are the principal natural fibrous materials, considered the most important renewable resource on earth, used as raw material for many industrial processes among them, for pulp and paper production, charcoal, and biofuels. For all these reasons, the study of molecular composition and biosynthesis of plant cell walls has been a matter of great interest for researchers over the past few years. In this context, a full-length cDNA fragment of Miox2 gene was cloned from Arabidopsis seedlings, using RT-PCR, with an open reading frame of 954 pb and a corresponding protein subunit molecular mass of 37 kDa. The deduced amino acid sequence of the cDNA showed a high degree of identity with myo-Inosytol oxygenases from other organisms. This cDNA was used for genetic transformation of tobacco, a model plant, which expressed either antisense or sense RNA. Transgenic homozygous tobacco plants, with either repression or constitutively expression of Miox2, were obtained with the number of copies varying from 1 to 7. Neither, the repression of the endogenous tobacco Miox genes or the constitutive expression of Miox2 gene, caused major impacts on plant development, leaf morphology or flowering time. Moreover, although statistically significant (P<0.05) changes in the arabinan and D-galacturonate contents were observed between transgenic and control lines, these results indicate that the modulation of the myo-Inositol pathway caused no major impacts also on cell wall polysaccharide biosynthesis. MenosCell walls are essential structures for plant development and growth. Apart from its biological functions, the polysaccharides that make cell walls (cellulose, hemicellulose and pectins) are the principal natural fibrous materials, considered the most important renewable resource on earth, used as raw material for many industrial processes among them, for pulp and paper production, charcoal, and biofuels. For all these reasons, the study of molecular composition and biosynthesis of plant cell walls has been a matter of great interest for researchers over the past few years. In this context, a full-length cDNA fragment of Miox2 gene was cloned from Arabidopsis seedlings, using RT-PCR, with an open reading frame of 954 pb and a corresponding protein subunit molecular mass of 37 kDa. The deduced amino acid sequence of the cDNA showed a high degree of identity with myo-Inosytol oxygenases from other organisms. This cDNA was used for genetic transformation of tobacco, a model plant, which expressed either antisense or sense RNA. Transgenic homozygous tobacco plants, with either repression or constitutively expression of Miox2, were obtained with the number of copies varying from 1 to 7. Neither, the repression of the endogenous tobacco Miox genes or the constitutive expression of Miox2 gene, caused major impacts on plant development, leaf morphology or flowering time. Moreover, although statistically significant (P<0.05) changes in the arabinan and D-galacturonate contents were o... Mostrar Tudo |
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Palavras-Chave: |
D-glucuronate; Hemicellulose; Myo-Inositol; Myo-Inositol oxygenase. |
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Categoria do assunto: |
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Marc: |
LEADER 02397naa a2200241 a 4500
001 1089050
005 2013-04-16
008 2012 bl uuuu u00u1 u #d
100 1 $aNASCIMENTO, D. D.
245 $aModulating MIOX2 expression in Nicotiana tabacum and impacts on genes involved in cell wall biosynthesis.
260 $c2012
520 $aCell walls are essential structures for plant development and growth. Apart from its biological functions, the polysaccharides that make cell walls (cellulose, hemicellulose and pectins) are the principal natural fibrous materials, considered the most important renewable resource on earth, used as raw material for many industrial processes among them, for pulp and paper production, charcoal, and biofuels. For all these reasons, the study of molecular composition and biosynthesis of plant cell walls has been a matter of great interest for researchers over the past few years. In this context, a full-length cDNA fragment of Miox2 gene was cloned from Arabidopsis seedlings, using RT-PCR, with an open reading frame of 954 pb and a corresponding protein subunit molecular mass of 37 kDa. The deduced amino acid sequence of the cDNA showed a high degree of identity with myo-Inosytol oxygenases from other organisms. This cDNA was used for genetic transformation of tobacco, a model plant, which expressed either antisense or sense RNA. Transgenic homozygous tobacco plants, with either repression or constitutively expression of Miox2, were obtained with the number of copies varying from 1 to 7. Neither, the repression of the endogenous tobacco Miox genes or the constitutive expression of Miox2 gene, caused major impacts on plant development, leaf morphology or flowering time. Moreover, although statistically significant (P<0.05) changes in the arabinan and D-galacturonate contents were observed between transgenic and control lines, these results indicate that the modulation of the myo-Inositol pathway caused no major impacts also on cell wall polysaccharide biosynthesis.
653 $aD-glucuronate
653 $aHemicellulose
653 $aMyo-Inositol
653 $aMyo-Inositol oxygenase
700 1 $aANDRADE, A.
700 1 $aLABATE, C. A.
700 1 $aVENEZIANO, L. M. T.
700 1 $aGUTMANIS, G.
700 1 $aBERTOLO, A. L.
700 1 $aBRAGATTO, J
773 $tBioenergia em Revista, Piracicaba, SP$gv. 2, n. 1, p. 60-82, 2012.
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| 5. |  | EBERHARDT, D.S.; STUKER, H. Avaliacao de herbicidas aplicados em pos-emergencia em solo inundado, no controle de plantas daninhas, em arroz irrigado. In: REUNIAO DA CULTURA DO ARROZ IRRIGADO, 21., 1995, Porto Alegre, RS. Anais... Porto Alegre,RS: IRGA, 1995. p.244-247.| Biblioteca(s): Epagri-Sede. |
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| 19. | | EBERHARDT, D. S.; NOLDIN, J. A. Dano ocasionado por sagitária (Sagittaria montevidensis) em arroz irrigado, sistema pré-germinado. In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 1.; REUNIÃO DA CULTURA DO ARROZ IRRIGADO, 23., 1999, Pelotas, RS. Anas... Pelotas, RS: Embrapa Clima Temperado, 1999. p. 578-580.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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