02397naa a2200241 a 450000100080000000500110000800800410001910000220006024501100008226000090019252016930020165300180189465300180191265300170193065300270194770000160197470000180199070000240200870000170203270000190204970000160206877300710208410890502013-04-16       2012    bl uuuu     u00u1 u    #d1 aNASCIMENTO, D. D.  aModulating MIOX2 expression in Nicotiana tabacum and impacts on genes involved in cell wall biosynthesis.  c2012  aCell walls are essential structures for plant development and growth. Apart from its biological functions, the polysaccharides that make cell walls (cellulose, hemicellulose and pectins) are the principal natural fibrous materials, considered the most important renewable resource on earth, used as raw material for many industrial processes among them, for pulp and paper production, charcoal, and biofuels. For all these reasons, the study of molecular composition and biosynthesis of plant cell walls has been a matter of great interest for researchers over the past few years. In this context, a full-length cDNA fragment of Miox2 gene was cloned from Arabidopsis seedlings, using RT-PCR, with an open reading frame of 954 pb and a corresponding protein subunit molecular mass of 37 kDa. The deduced amino acid sequence of the cDNA showed a high degree of identity with myo-Inosytol oxygenases from other organisms. This cDNA was used for genetic transformation of tobacco, a model plant, which expressed either antisense or sense RNA. Transgenic homozygous tobacco plants, with either repression or constitutively expression of Miox2, were obtained with the number of copies varying from 1 to 7. Neither, the repression of the endogenous tobacco Miox genes or the constitutive expression of Miox2 gene, caused major impacts on plant development, leaf morphology or flowering time. Moreover, although statistically significant (P&lt0.05) changes in the arabinan and D-galacturonate contents were observed between transgenic and control lines, these results indicate that the modulation of the myo-Inositol pathway caused no major impacts also on cell wall polysaccharide biosynthesis.  aD-glucuronate  aHemicellulose  aMyo-Inositol  aMyo-Inositol oxygenase1 aANDRADE, A.1 aLABATE, C. A.1 aVENEZIANO, L. M. T.1 aGUTMANIS, G.1 aBERTOLO, A. L.1 aBRAGATTO, J  tBioenergia em Revista, Piracicaba, SPgv. 2, n. 1, p. 60-82, 2012.