01448naa a2200157 a 450000100080000000500110000800800410001910000110006024500800007126000090015152009860016065300210114665300210116765300220118877300800121010847252012-05-03       2000    bl uuuu     u00u1 u    #d1 aEpagri  aHormonal and histological studies related to in vitro banana bud formation.  c2000  aShoot apices of Musa subgroup AAA 'Grande Naine' were used for in vitro culture establishment. The endogenous horrnone leveis and their effects on bud forrnation were evaluated during a 75-day period. Cytokinins, IAA and ABA were separated by HPLC and quantified by means of ELISA. Enzymatic degradation of IAA was determined by the colorimetric method. Explants were maintained on establishment medium for 60 days. The endogenous cytokinins were higher in the basal portion of the explant. Subculture to proliferation medium (65 to 75 days) resulted in a substantial increase of cytokinins in the basal portion and in a decline in the apical portion. 2iP was the predominant cytokinin in the tissue. The endogenous levei of IAA and the IAA/cytokinin ratio decreased after the 65th day of culture. The levei of ABA was reduced from the time of inoculation up to the 75th day of culture. Histological analysis indicated that buds forrned at the leaf base at the 65th day of culture.  aBud regeneration  aMicropropagation  aMusa subgroup AAA  tPlant Cell, Tissue and Organ Culture, Netherlandsgv. 63, p. 187-192, 2000.