01470naa a2200241 a 450000100080000000500110000800800410001910000190006024501280007926000090020752006830021665300440089965300370094365300360098065300140101670000210103070000200105170000170107170000210108870000150110970000170112477300870114111300182020-10-07       2020    bl uuuu     u00u1 u    #d1 aMANENTI, D. C.  aSerological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa Catarina.h[electronic resource]  c2020  aThe Apple Germplasm Bank (AGB) of Santa Catarina Agricultural Research and Rural Extension Company - Epagri, AGB-Epagri, is the largest of the genus Malus in Brazil. Twenty-eight main accessions of this bank were virus screened through DAS-ELISA, RT-PCR and IC-RT-PCR during two consecutive reproductive cycles, and each accession showed latent mixed infection by at least two species, among ASGV, ASPV and ACLSV. The combined use of diagnostic methods helped overcome inconsistencies commonly found in apple virus detection and was shown essential for the AGB-Epagri can be safely used as a source of genetic variability and for the exchange of virus-free propagative material.  aapple chlorotic leaf spot virus (ACLSV)  aapple stem grooving virus (ASGV)  aapple stem pitting virus (ASPV)  aMalus spp1 aHAWERROTH, M. C.1 aBRANCHER, T. L.1 aSCHUH, F. S.1 aKVITSCHAL, M. V.1 aNICKEL, O.1 aSOUTO, E. R.  tBrazilian Archives of Biology and Technology, Curitibagv. 63, p. e20190272, 2020.