03224naa a2200241 a 450000100080000000500110000800800410001910000200006024501660008026000090024652024540025565300150270965300130272465300220273765300140275965300110277370000160278470000160280070000220281670000170283870000230285577301040287811290252019-10-25       2019    bl uuuu     u00u1 u    #d1 aSOUZA, D. S. M.  aINFLUENCE OF FAECAL CONTAMINATION FROM THE CAMBORIÚ RIVER ON THE MICROBIOLOGICAL QUALITY OF WATER IN A BIVALVE SHELLFISH PRODUCTION AREA.h[electronic resource]  c2019  aThe state of Santa Catarina is a major shellfish producer in Brazil, responsible for 95% of the national production of bivalves such as Crassostrea gigas oysters and Perna perna mussels. A significant part of the shellfish production areas (SPAs) in this state is located close to rivers draining urban areas lacking sewage collection and treatment systems (SCTS). This is a concern since bivalves are filter feeders and may accumulate human pathogens in their tissues, becoming vectors of diseases. One of these SPAs is located ~1.5 km far from the Camboriu River (CR) mouth. The river catchment covers two municipalities with very different profiles: Camboriú, upstream, has agriculture and industry as main activities, an estimated human population of 80,830 and no coverage with STCS; and Balneário Camboriú (BC), downstream, has tourism as its main activity, hosting every year around 3 million tourists during the summer. BC has an estimated baseline population of 138,730, and high STCS coverage, 94%. The aim of this study was to evaluate the prevalence of faecal indicator bacteria and enteric viruses along the CR and analyze the impact of the river discharge on the microbiological quality of water in the adjacent SPA. The levels of Thermotolerant coliforms (TC), Human Adenovirus (HAdV), Hepatitis A virus (HAV), Norovirus (NoV) genogroups I and II were monitored fortnightly during one year at 5 sites: 4 points along the CR and 1 at the SPA. TC were quantified by culture and the enteric viruses by qPCR. A gradient in the TC levels was observed, with the highest results obtained in the most upstream point (geometric mean-GM= 3.9*104 MPN.100 mL-1) and decreasing levels were detected in the points located downstream. The TC levels in the SPA were the lowest (GM= 4.5 and 90th percentile = 29 MPN.100 mL-1), within the legal limits for shellfish direct consumption without post-harvesting treatment (Approved area under US criteria). NoV and HAV were not detected, while HAdV was detected exclusively in points within the CR. The genome of this virus was detected in one to three samples among the 24 samples collected per site. The study confirms the faecal contamination of the CR and suggests that the pollution arising from this river does not affect significantly the microbiological water quality in the SPA located in the coast of BC. Modelling studies are needed to further understand the pollution dynamics in this area.  aAdenovirus  aBacteria  aHepatitis A virus  aNorovirus  aOyster1 aMORESCO, V.1 aFONGARO, G.1 aBARARDI, C. R. M.1 aSOUZA, R. V.1 aGARBOSSA, L. H. P.  tIn: CONGRESSO BRASILEIRO DE VIROLOGIA, 30., 2019, Cuiabá. Resumos... Novo Hamburgo, RS: SBV, 2019.