02495naa a2200193 a 450000100080000000500110000800800410001910000150006024501380007526000090021352018210022265300210204365300190206465300220208370000190210570000150212470000180213977301440215711288562019-09-06       2019    bl uuuu     u00u1 u    #d1 aARAUJO, L.  aSymptoms of bacterial shoot blight associated with Pseudomonas syringae pv. syringae in apple trees in Brazil.h[electronic resource]  c2019  aDiseases of fruit trees caused by Pseudomonas syringae are a major concern in fruit producing areas worldwide. In Brazil, apple plants showing bacterial symptoms were observed in a commercial orchard in S??o Joaquim, SC, during the summer of 2016. Thus, the objective of the present study was to identify the causal organism of those symptoms. Isolation from the affected tree samples was performed on King?s B (KB) medium. After 48 hours at 28 ??C, most of bacterial isolates formed round, shiny, gray-white colonies producing green fluorescent pigment. The Gramnegative bacterium belongs to the group Ia (LOPAT +???+). Detached leaves of cv. Gala and immature fruits of cv. Braeburn, Fuji, Kinkas, and Pink Lady of apple, inoculated with a bacterial suspension, showed intense necrosis and decay, respectively, four days after inoculation (d.a.i.). Similarly, 1.5-year-old apple plants cv. Gala with Marubakaido + M.9 interstock, grown in plastic pots with 1 kg of substrate, kept in greenhouse, presented wilting, leaf blight, dark xylem tissue visible along the branch length, and red-rust bacterial ooze, 30 d.a.i. The bacterium with the same characteristics was readily re-isolated from the different infected tissues. LOPAT tests indicated the isolates belonged to P. syringae pv. syringae. The taxonomic positioning of isolate was determined using Genome-to- Genome Distance Calculator, by in silico DNA-DNA hybridization and showed 93.9% similarity with P. syringae strain 31R1.One isolate was deposited in the IBSBF Culture Collection (Instituto Biol??gico, SP, Brazil) and cataloged as #IBSBF 3261. Genome sequence was deposited in the Genbank, accession number: QOSP00000000. To our knowledge this is the first report of P. syringae pv. syringae causing bacterial canker in commercial orchards in Brazil.  abacterial canker  abacterial ooze  ainternal necrosis1 aCARDOZA, Y. F.1 aDUARTE, V.1 aMORAES, M. G.  tIn: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife, PE. Resumos... Recife, PE: Sociedade Brasileira de Fitopatologia, 2019. p. 92