02408naa a2200277 a 450000100080000000500110000800800410001910000190006024501300007926000090020952015230021865300160174165300210175765300110177870000160178970000210180570000180182670000240184470000140186870000240188270000170190670000200192370000210194370000170196477301490198111285552019-07-03       2019    bl uuuu     u00u1 u    #d1 aLENS, H. H. M.  aHylocereus undatus SEEDS PROTECTS CELL MEMBRANE FROM LIPOPEROXIDATION INDEPENDENTLY OF IRON CHELATION.h[electronic resource]  c2019  aThe objective of this work was to evaluate the antioxidant profile of the bark, pulp and seed of the white pitaya specie, Hylocereus undatus, through the verification of the ability to protect biological membranes. The fruits of H. undatus (n = 7) were processed to obtain a sample of the bark, pulp and seed, separately. Hepatic microsomes were also obtained from healthy rats for lipoperoxidation assay. The test groups were divided into control (microsome + phosphate buffer solution (PBS) + tert-butil), oxidized microsome (microsome + FeCl3 + ascorbic acid + PBS + tert-butil), pitaya with control microsome (pitaya + microsome + PBS + tert-butil) and pitaya with oxidized microsomes (microsome + FeCl3 + ascorbic acid + tert-butil). The pretreatment of the microsomes was performed with a sample of bark, pulp and pitaya seed. After 12 hours of incubation at 37 ?? C, oxidative stress was evaluated by chemiluminescence. To confirm the antioxidant activity of pitaya, a spectrophotometric evaluation of Fe3+ complex formation was performed.Our results show that, based on the significant increase in lipoperoxidation of the oxidized microsome group when compared to the control group (p &lt0.001), only the Hylocereus undatus seed sample was able to significantly prevent the microsome lipoperoxidation (p &lt0.001). In evaluating whether the antioxidant protection of pitaya would be related to the iron chelation of the lipoperoxidation system, we showed that there is no formation of pitaya complex with Fe3+.  aAntioxidant  aoxidative stress  apitaya1 aLONE, A. B.1 aBLEGNISKI, F. P.1 aBORGES, F. H.1 aBANIN-HIRATA, B. K.1 aPANIS, C.1 aTAKAHASHI, L. S. A.1 aFARIA, R. T.1 aCECCHINI, A. L.1 aVICTORINO, V. J.1 aCECCHINI, R.  tIn: INTERNATIONAL SYMPOSIUM OF EXPERIMENTAL PATHOLOGY, 2., 2019, Londrina. Abstracts... Londrina: Universidade Estadual de Londrina, 2019. p. 79