02853naa a2200241 a 450000100080000000500110000800800410001910000130006024501080007326000090018152020630019065300230225365300410227665300180231765300190233570000130235470000180236770000180238570000140240370000180241770000230243577301530245811280452018-11-27       2018    bl uuuu     u00u1 u    #d1 aBOGO, A.  aPestalotiopsis spp. causing necrotic leaf spot on grapevines in southern Brazil.h[electronic resource]  c2018  aMany species of the genus Pestalotiopsis are common in tropical and temperate regions and are often isolated as endophytic, saprophytic or pathogenic. There are few reports of Pestalotiopsis spp. as a primary disease in grapevine, especially in typical symptoms of anthracnose. Recently, Pestalotiopsis has been reported in South Korea, Australia and the USA causing grape diseases in branches, woody tissue, fruits, flowers and leaves, being the second most commonly isolated fungus of grapevine cancers in Texas/USA. The present study aimed to characterize the occurrence of Pestalotiopsis spp. causing anthracnose symptoms in grape leaves, branches and fruits at southern Brazil. Samples of grapevine plant tissues (Vitis vinifera L.) with typical symptoms of anthracnose were collected in 21 municipalities of the three southern Brazilian states from November 2013 to March 2015. The pathogen was isolated in potato agar dextrose culture medium and morphological characterization of the isolates as size of the conidia (width and length), size and number of apical appendages, growth rate index and final colony diameter were assessed. The pathogenicity of the isolates were tested on grape leaves and fruits. Data were submitted to analysis of variance and the means compared by the Scott Knott test at 5% probability of error and the isolates grouped by multivariate analysis. The ITS genomic region of one representative isolates was sequenced. The isolates showed morphological variation and were distributed into five groups. Comparison of the nucleotide sequence obtained for the isolates through BLAST and phylogenetic analysis confirmed the presence of Pestalotiopsis spp., suggesting the presence of more than one species of the genus. The pathogenicity test confirmed the ability of the isolates to cause grape leaf lesions and fruit rot. This study represents the first report of the identification and characterization of the genus  Pestalotiopsis causing diseases in grapevine in southern Brazil using morphological and molecular approaches.  aITS genomic region  amorphological and molecular features  apathogenicity  aVitis vinifera1 aPIVA, C.1 aWELTER, L. J.1 aNODARI, R. O.1 aGOMES, B.1 aMICHELSKI, V.1 aKLABUNDE, G. H. F.  tIn: INTERNATIONAL HORTICULTURAL CONGRESS, 30., 2018, Turquia. Abstracts... Turquia: International Society for Horticultural Science, 2018. p. 27-28.