01948naa a2200277 a 450000100080000000500110000800800410001910000130006024501930007326000090026652010670027565300080134265300300135065300330138065300110141365300220142465300090144670000210145570000210147670000190149770000170151670000190153370000170155270000240156977300770159311271442018-03-02       2017    bl uuuu     u00u1 u    #d1 aBACH, E.  aDetection of misidentification of species from the Burkholderia cepacia complex and description of a new member, the soil bacteria Burkholderia catarinensis sp. nov.h[electronic resource]  c2017  aThe correct identification of bacteria from the Burkholderia cepacia complex (Bcc) is crucial for epidemiological studies and treatment of cystic fibrosis infections. However, genome-based identification tools are revealing many controversial Bcc species assignments. The aim of this work is to re-examine the taxonomic position of the soil bacterium B. cepacia 89 through polyphasic and genomic approaches. recA and 16S rRNA gene sequence analysis positioned strain 89 inside the Bcc group. However, based on the divergence score of seven concatenated allele sequences, and values of average nucleotide identity, and digital DNA:DNA hybridization, our results suggest that strain 89 is different from other Bcc species formerly described. Thus, we propose to classify Burkholderia sp. 89 as the novel species Burkholderia catarinensis sp. nov. with strain 89T (=DSM 103188T = BR 10601T) as the type strain. Moreover, our results call the attention to some probable misidentifications of Bcc genomes at the National Center for Biotechnology Information database.  aANI  aBurkholderia catarinensis  aBurkholderia cepacia complex  agenome  amisidentification  aMLSA1 aSANT'ANNA, F. H.1 aPASSOS, J. F. M.1 aBALSANELLI, E.1 aBAURA, V. A.1 aPEDROSA, F. O.1 aSOUZA, E. M.1 aPASSAGLIA, L. M. P.  tPathogens and Diseases, Oxford, Reino Unidogv. 6, n. 75, p. 1-24, 2017.