02719naa a2200241 a 450000100080000000500110000800800410001910000250006024501730008526000090025852019200026765300270218765300300221465300240224465300120226870000210228070000190230170000210232070000270234170000170236870000210238577300710240611270262018-01-31       2017    bl uuuu     u00u1 u    #d1 aGON??ALVES, G. L. P.  aCompounds from Duguetia lanceolata St.- Hil. (Annonaceae) bioactiveagainst Zabrotes subfasciatus (Boheman) (ColeopterabChrysomelidae:Bruchinae).h[electronic resource]  c2017  aThe variety of chemical compounds produced by members of Annonaceae exhibiting wide activity againstpest organisms makes this family an interesting source for insecticidal compound identification. Accord-ingly, this study first aimed to evaluate the lethal and sublethal effects of ethanolic extracts of differentparts of Duguetia lanceolata St.-Hil. against Zabrotes subfasciatus (Boheman) (Coleoptera: Chrysomeli-dae: Bruchinae), the primary pest of stored beans in tropical regions. Based on a screening bioassay,the ethanolic extract from D. lanceolata leaves was selected for chemical partitioning due to its highbioactivity against the weevil. The hexanic partition of the ethanolic leaf extract caused 98% mortalityof Z. subfasciatus adults in a residual contact bioassay and completely eliminated the number of eggsper sample. Chromatography subfraction Flh2-5, a hexanic partition of D. lanceolata leaves, resulted in100% mortality of Z. subfasciatus adults. An aromatic compound (2,4,5-trimethoxystyrene) and a mixtureof sterols [(campesterol (8.44%) + stigmasterol (12.37%) + sitosterol (79.19%)] were isolated from subfrac-tion Flh2-5 using various chromatographic procedures. The aromatic compound resulted in 60% weevilmortality, and the steroid mixture applied at 75 mg kg−1promoted 28% mortality; in contrast, the positivecontrol (K-Obiol??2P, deltamethrin 2 g a.i./kg) at 375 mg kg−1resulted in 100% mortality. Moreover, both2,4,5-trimethoxystyrene and K-Obiol??2P completely prevented oviposition on bean samples, demon-strating that, similar to a deltamethrin-based formulation, 2,4,5-trimethoxystyrene is able to act as a grainprotector. Therefore, the aromatic compound and mixture of isolated sterols from D. lanceolata may besuitable management tools of stored-product pests and promising sources of insecticidal molecules withboth lethal and sublethal effects on Z. subfasciatus.  aBotanical insecticides  aChromatography procedures  aMexican bean weevil  aSterols1 aDOMINGUES, V. C.1 aRIBEIRO, L. P.1 aFERNANDES, J. B.1 aFERNANDES, M. F. G. A.1 aFORIM, M. R.1 aVENDRAMIM, J. D.  tIndustrial Crops and Products, Amsterd??gv. 97, p. 360-367, 2017.