02373naa a2200301 a 450000100080000000500110000800800410001910000160006024501840007626000090026052014610026965300220173065300170175265300250176965300250179465300210181965300160184070000210185670000210187770000170189870000160191570000200193170000150195170000180196670000190198470000230200377300450202611262432017-05-24 2016 bl uuuu u00u1 u #d1 aPEREIRA, S. aAutochthonous probiotic Lactobacillus sp. in the diet of bullfrog tadpoles Lithobates catesbeianus improves weight gain, feed conversion and gut microbiota.h[electronic resource] c2016 aDietary supplementation with probiotics in animal production is an alternative to antibiotics. In frog culture, studies involving native strains of probiotic bacteria and their effects on the performance and intestinal histology of farmed animals are scarce. Therefore, this study aimed to evaluate a diet supplemented with Lactobacillus sp. in tadpoles of Lithobates catesbeianus. This randomized test was performed with two dietary treatments: non-supplemented control diet and diet supplemented with Lactobacillus sp., with nine replications. The growth performance of Lactobacillus sp., including its bacterial enzymatic activity and stability in feed, as well as colonization and histology of the intestinal tract, was evaluated after 42 days of experimentation. Animals fed with a supplemented diet showed higher weight gain and concentration of lactic acid bacteria in the gut and lower feed conversion. No significant difference was observed in survival, total heterotrophic bacterial count or histological change in the gut between the two treatments. The Lactobacillus sp. strain was able to colonize the intestinal tract and feed and remain at a high concentration of 107 and 106 CFU g−1, respectively. It produced several enzymes, which might have contributed to the greater weight gain and lower feed conversion in the supplemented animals, thus demonstrating its probiotic potential for use as a dietary supplement in bullfrog tadpoles. aenzyme production afrog culture aintestinal histology alactic acid bacteria amelanomacrophage aweight gain1 aJERÔNIMO, G. T.1 aMARCHIORI, N. C.1 aOLIVEIRA, H.1 aOWATARI, M.1 aJESUS, G. F. A.1 aGARCIA, P.1 aVIEIRA, F. N.1 aMARTINS, M. L.1 aMOURIÑO, J. L. P. tAquaculture Nutrition, New Jersey, 2016.