02386naa a2200181 a 450000100080000000500110000800800410001910000170006024501460007726000090022352016870023265300250191965300200194465300350196470000200199970000200201977301650203911258832016-12-16       2016    bl uuuu     u00u1 u    #d1 aCanale, M.C.  aLATENCY OF 'CANDIDATUS LIBERIBACTER ASIATICUS' IN ITS PSYLLID VECTOR, Diaphorina citri KUWAYAMA (HEMIPTERAbLIVIIDAE).h[electronic resource]  c2016  aInformation on transmission parameters of vector-borne plant pathogen is mandatory for knowledge of insect-pathogen relationship and disease epidemiology to improve disease management. ?Candidatus Liberibacter asiaticus? (Las), the major pathogen associated with citrus Huanglongbing (HLB), requires a latent period between its acquisition from a source plant and the transmission to a new plant host by the psyllid vector Diaphorina citri. Latency of Las in D. citri reported in the literature is inconsistent and ranges from 1 to 25 days. We examined the mean latent period (LP50), i.e. time after acquisition when 50% of the individuals can inoculate, of Las in D. citri. Third-instar psyllid nymphs and 1-week old adults were allowed to an acquisition access period (AAP) on infected citrus, and submitted to sequential 48-h inoculation access periods (IAPs), in a series of healthy citrus seedlings, at 25??C. LP50 was estimated by transforming the cumulative percentage of first transmissions to a log-probit scale. Latency is 16.5 and 18 days for psyllids that acquired Las as nymphs and adults, respectively. Inoculation events were intermittent, randomly distributed over the IAPs, and more frequent after acquisition by nymphs. The long latency of Las in D. citri is an indirect evidence for circulation of bacteria in insect body and reinforces the persistent propagative relationship with the vector. Regarding management implications, the latency period represents a ?gap? following bacterial acquisition during which psyllids cannot transmit, and thus vector control can be applied in order to prevent pathogen spread. Financial support: CNPq/Brazil; CRDF/Florida/USA.  aAsian citrus psyllid  acitrus greening  aprokaryote-vector relationship1 aTOMASETO, A. F.1 aLOPES, J. R. S.  tIn: WORKSHOP BRASILEIRO DE EPIDEMIOLOGIA DE DOEN??AS DE PLANTAS, 5., 2016, Cruz das Almas, BA. Resumos... Cruz das Almas: Embrapa Mandioca e Fruticultura, 2016.