01773naa a2200217 a 450000100080000000500110000800800410001910000190006024501620007926000090024152010850025065300250133565300270136065300100138770000170139770000210141470000230143570000170145870000210147577300590149611255662016-09-26 2016 bl uuuu u00u1 u #d1 aBETTONI, J. C. aFREE CULTURE MEDIA OF GROWTH REGULATORS ON MICROPROPAGATION OF GRAPEVINE (VITIS LABRUSCA L.) ?BORD??? CULTIVAR THROUGH NODAL SEGMENTS.h[electronic resource] c2016 aThe micropropagation is an important biotechnological tool for obtaining and maintaining mother grapevine plants with high quality plant health. The objective was to evaluate the establishment and multiplication in vitro and ex vitro acclimatization of grapevine cultivate Bord?? in different culture media without adding growth regulators. Grapevine nodal segments were cultured in five culture media formulations without adding growth regulators. It was evaluated the number of leaves and roots, length of roots and shoots, replication rate, relative chlorophyll index, percentage of regenerated and rooted plants, dry biomass of shoot, root and total plants grown in vitro and after acclimatization. In vitro propagation of grapevine Bord?? through nodal segments provided high rates of regeneration and rooting. High survival rates were obtained in the acclimatization of Bord??. Considering all the variables, culture media Roubelakis and Zlenko showed the best growth rates and development for shoots and roots, and in vitro multiplication rate of Bord?? grapevine cultivar. ain vitro propagation avegetative propagation aVitis1 aCOSTA, M. D.1 aGARDIN, J. P. P.1 aKRETZSCHMAR, A. A.1 aSOUZA, J. A.1 aPASSOS, J. F. M. tEvid??ncia, Chapec??, SCgv. 16, n. 1, p. 59-70, 2016.