02386naa a2200217 a 450000100080000000500110000800800410001910000200006024501500008026000090023052016640023965300150190365300230191865300210194165300240196265300310198670000170201770000200203470000270205477300870208111254082016-09-08 2016 bl uuuu u00u1 u #d1 aROCHA, G. A. F. aIdentification and mapping of resistance genes to Phakopsora pachyrhizi in soybean (Glycine max L.) accession PI 594767-A.h[electronic resource] c2016 ahe goal of this study was to study resistance inheritance in the soybean (Glycine max L.) accession PI 594767-A to the Phakopsora pachyrhizi isolate PPUFV02, and map the resistance gene(s) identified using microsatellite markers. Crosses between PI 594767-A and the susceptible cultivar ?Conquista? gave rise to the segregating subpopulations 26C-2 and 26C-5, which in the F2 generation were evaluated for their reactions to PPUFV02. In addition, analyses with microsatellite markers linked to the Rpp1-Rpp5 loci were also performed. The segregation pattern obtained in 26C-2 revealed that resistance was governed by a recessive gene; a 1:2:1 segregation pattern was observed in 26C-5, indicating control by a gene with partial dominance. This variability may have been caused because environmental conditions, particularly temperature, when 26C-5 was assessed were unfavorable for pathogen development, allowing the phenotypic expression of heterozygous alleles in PI 594767-A. A resistance gene was located in the soybean linkage group G, in the genomic region between Sct_187r2 and Sat_064 that contains the Rpp1 locus. Resistance in PI 594767-A is probably conferred by a new Rpp1 gene allele, because this accession has a haplotype for Sct_187r2 and Sat_064, which differs from haplotypes of accessions that also contain resistance alleles that map the Rpp1 locus. The use of Sct_187r2 and Sat_064 will facilitate the introgression of the resistance allele from PI 594767-A and its pyramiding with other resistance genes into genotypes with superior agronomic characteristics, in order to obtain cultivars with broad-spectrum resistance to P. pachyrhizi. aAsian rust aDisease resistance aGenetic analysis aGenetic inheritance aResistance genetic mapping1 aALVES, D. P.1 aOLIVEIRA, J. C.1 aBROMMONSCHENKEL, S. H. tGenetics and Molecular Research , Ribeirão Preto, SPgv. 15, n. 3, p. 1-15, 2016.