02689naa a2200217 a 450000100080000000500110000800800410001910000190006024501380007926000090021752019430022665300150216965300260218465300170221070000190222770000210224670000180226770000230228570000210230877301420232911234092015-03-20       2014    bl uuuu     u00u1 u    #d1 aBICALHO, K. U.  aBioguided phytochemical study of Annona mucosa searching for natural insecticides against Sitophillus zeamais.h[electronic resource]  c2014  aThe search for natural products with insecticidal potential to substitute synthetic insecticides in pest management has widely increased due the low toxicity and fast degradation of the biopesticides. In this work, Annona mucosa was studied searching for compounds to control maize weevil, a serious agricultural pest that causes large losses in the Brazilian crop production.The phytochemical study of A. mucosa was performed based on the bioassays results against S. zeamais, where only the bioactive fractions were studied. For the bioassays, were evaluated the mortality of weevils submitted to the treatment with the samples and the lethal concentrations for the most active ones, that were studied through chromatographic techniques. Initially were evaluated extracts from leaves, branches and seeds from A. mucosa. The bioassay revealed being the seed extract the most active causing 100% of mortality of weevils at 1500 ppm and LC50 of 288 ppm. After a liquid-liquid partition of this extract, the hidroalcoholic fraction, rich in acetogenins, showed 94% of mortality at 288 ppm. Among the subfractions, the most active one, tested at 58 ppm, presented mortality higher than 80% and was studied through HPLC, which lead to the isolation of 4 acetogenins, rolliniastatin-1 and 2, jimenezin and a novel acetogenin. Rolliniastatin-1, the majority compound from this fraction, was tested and resulted in 50% of mortality at 58 ppm, and was observed loss of motor coordination symptoms in all subjects tested. The results showed the great insecticide potential of the species A. mucosa and of the acetogenins. The concentration used in rolliniastatin-1 bioassay is near of those values for commercial insecticides registered at Brazil?s Ministry of Agriculture, highlighting the possibility of using this molecule as a bioinsecticide or as ?lead strucutre? for the development of new insecticides that are less toxic and more specific.  aacetogenin  aBotanical insecticide  amaize weevil1 aRIBEIRO, L. P.1 aVANDRAMIM, J. D.1 aVIEIRA, P. C.1 aSILVA, M. F. G. F.1 aFERNANDES, J. B.  tIn: CONGRESS OF THE LATIN AMERICAN ASSOCIATION OF CHEMICAL ECOLOGY, 3., 2014, Bogot??, Colombia. Anais... Bogot??, Colombia: ALAEQ, 2014.