02534naa a2200253 a 450000100080000000500110000800800410001910000220006024501110008226000090019352017080020265300160191065300150192665300250194165300110196665300230197770000180200070000170201870000210203570000150205670000150207170000190208677301750210511233732015-03-18 2015 bl uuuu u00u1 u #d1 aMARTINI, L. F. D. aEffect of Planting Time and Bispyribac-sodium on Gene Expression of Rice Seedlings.h[electronic resource] c2015 aThe weed control improvement on modern rice crop production allowed to shift the planting time to early spring, which is very important management practice to achieve high yield. However early planting time exposes the rice seedlings to cold stress, impairing the crop establishment and also herbicide selectivity. The objective of this study was to evaluate the effect of cold stress combined with bispyribac-sodium application on molecular and physiological patterns of rice seedlings. A greenhouse experiment was conducted in 2010 at Universidade Federal de Pelotas, RS, Brazil. The treatments were composed by factorial design: factor A - planting times: late September and early November; factor B - bispyribac-sodium application and untreated check. Injury at 7, 14, 21 and 28 days after treatment (DAT); lipid peroxidation and total phenols at 7 DAT and APX, CAT, SOD, OsDREB1A, OsFAD8, OsCDPK13, CYP72A21, OsGSTL2 and OsGSTU10 gene expression at 12, 24, 48 hours after treatment HAT and 14 DAT were evaluated. Early planting time promoted greater bispiribac-sodium injury on rice seedlings. Lipid peroxidation and total phenols accumulation was stimulated by low temperature, however were not affected by bispyribac-sodium application. SOD, CAT, APX, OsDREB1A, OsCDPK13, CYP72A2 and OsGSTL2 were responsive to low temperature and presented overexpression when exposed to herbicide, evidencing cross-talk between those two stress agents. OsFAD8 and OsGSTU10 presented significant expression under cold, however not responsive to herbicide. These results supports that cold affect not only herbicide metabolism but also on antioxidant machinery in order to cope secondary effect promoted by them. acold stress across-talk aherbicide metabolism ainjury alipid peroxidation1 aNOLDIN, J. A.1 aAVILA, L. A.1 aSCHAEDLER, C. E.1 aBORGES, C.1 aZIMMER, P.1 aAGOSTINETO, D. tIn: ANNUAL MEETING OF THE WEED SCIENCE SOCIETY OF AMERICA, 55., 2015, Lexington, Estados Unidos. Proceedings... Lawrence , KS, EUA: Weed Science Society of America, 2015.