02099naa a2200205 a 450000100080000000500110000800800410001910000190006024501240007926000090020352013900021265301090160270000190171170000190173070000180174970000190176770000200178670000210180677300660182711224482014-12-01 2014 bl uuuu u00u1 u #d1 aZANARDI, O. Z. aBioactivity of a matrine-based biopesticide against four pest species of agricultural importanceh[electronic resource] c2014 aThe bioactivity of a matrine-based biopesticide was assessed against four pest species (Diaphorina citri, Panonychus citri, Sitophilus zeamais and Spodoptera frugiperda) of agricultural importance in different systems of production. For this purpose, we conducted bioassays under laboratory, semi-field (greenhouse) and field (commercial farming) conditions. In our laboratory bioassay, the matrine-based biopesticide caused lethal and sublethal effects on all the studied pest species. However, its acaricidal activity against P. citri was between ~ 10 and 100 times more pronounced than its insecticidal activity (on D. citri, S. zeamais and S. frugiperda). In the greenhouse, the acaricidal activity of this formulation was similar to that of spirodiclofen (the positive control). Despite its high efficacy, the biopesticide showed a short residual effect (~ 1 day) in our semi-field bioassay. In commercial farm of citrus (with natural infestation of P. citri and Eutetranychus banksi), this biopesticide formulation (at 150 ppm) showed high efficacy (~ 90%) in the population reduction of the mites, with its acaricidal activity similar to that of spirodiclofen. The matrine-based biopesticide is a useful component for crop protection where the studied pest species occur, mainly in ecological systems of food production, which are in need of efficient tools for pest management. aBotanical pesticide alkaloids Diaphorina citri Panonychus citri Sitophilus zeamais Spodoptera frugiperda1 aRIBEIRO, L. P.1 aANSANTE, T. F.1 aSANTOS, M. S.1 aBORDINI, G. P.1 aYAMAMOTO, P. T.1 aVENDRAMIM, J. D. tCrop Protection, Guildfordgv. 67, n. 2015, p. 160-167, 2014.