01516naa a2200193 a 450000100080000000500110000800800410001910000160006024501290007626000090020552007470021465300110096165300210097265300130099370000200100670000160102670000170104277302630105911192602013-10-04       2013    bl uuuu     u00u1 u    #d1 aPEREIRA, A.  aDirect Colony PCRban alternative for the amplification of DNA sequences from Mycosphaerella species.h[electronic resource]  c2013  aObtaining DNA of good quality for PCR or sequencing is the first step in most of the current molecular biology applications. Many protocols are cumbersome to apply and use toxic products. We compared a method commonly used in many laboratories with a more simplified method ? direct colony PCR ? that does not use any hazardous reagents and equipment. We employed species belonging to the Mycosphaerella complex, and used the DNA for PCR and sequencing. The results showed that the direct colony PCR generated the expected band sizes when the primers ITS1-5.8s-ITS2, and for specific sequences for M. fijiensis or M. musicola were used. Furthermore, the sequencing of the ITS-amplified region showed a 100% similarity between the two methods.  aBanana  aMolecular marker  aSigatoka1 aTCACENCO, F. A.1 aHINZ, R. H.1 aSILVA, C. M.  tIn: REUNIÃO INTERNACIONAL DA ASSOCIAÇÃO PARA COOPERAÇÃO EM PESQUISA E DESENVOLVIMENTO INTEGRAL DAS MUSÁCEAS (BANANA E PLÁTANO) - ACORBAT BRASIL 2013, 20., 2013, Fortaleza, CE. Anais... Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2013. p. 288.