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| 3. |  | SILVA, C. M.; PAULINO, E. C.; PEREIRA, A.; HINZ, R. H.; LICHTEMBERG, L. A.; TCACENCO, F. A.; STADNIK, M. J. Genotipagem de cultivares de bananeira do banco de germoplasma da Epagri por RAPD. Agropecuária Catarinense , Florianópolis, v. 27, n. 2, p. 94-97, 2014.| Biblioteca(s): Epagri-Sede. |
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| 4. | | CUNHA, C. M. S.; HINZ, R. H.; PEREIRA, A.; TCACENCO, F. A.; PAULINO, E. C.; STADNIK, M. J. A SCAR marker for identifying susceptibility to Fusarium oxysporum f. sp. cubense in banana. Scientia Horticulturae, British Columbia, v. 191, p. 108-112, 2015.| Biblioteca(s): Epagri-Sede. |
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| Registros recuperados : 4 | |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
08/09/2015 |
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Data da última atualização: |
08/09/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - B |
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Autoria: |
CUNHA, C. M. S.; HINZ, R. H.; PEREIRA, A.; TCACENCO, F. A.; PAULINO, E. C.; STADNIK, M. J. |
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Título: |
A SCAR marker for identifying susceptibility to Fusarium oxysporum f. sp. cubense in banana. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Scientia Horticulturae, British Columbia, v. 191, p. 108-112, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Fusarium wilt (also known as Panama disease) caused by Fusarium oxysporum Schlechtend.
f. sp. cubense (E.F. Smith) W.C. Snyder & H.N. Hansen (Foc) is an endemic disease in all banana (Musa
spp. L.) producing areas. The use of resistant cultivars is the recommended method for the disease
control. The development of resistant or tolerant cultivars is expensive and time-consuming since the
results must be confirmed by many years of field evaluation. In this situation, DNA markers show a
great potential to improve the efficiency and precision of conventional plant breeding. Thus, the
objective of this work was to develop a SCAR marker able to discriminate between resistant and
susceptible genotypes to Foc infection. Using genomic DNA from resistant or susceptible genotypes
and 78 10-mer arbitrary primers, one RAPD band associated to susceptibility was selected and used to
generate the SCAR marker SuscPD-F/SuscPD-R. This marker was validated on 28 banana cultivars
which were resistant or susceptible to infection by Foc. The results indicated a high degree of
specificity of the marker, which was able to discriminate between the two contrasting groups
(resistant or susceptible). The discriminatory power of the new marker was 93%. |
|
Palavras-Chave: |
molecular marker; Panama disease. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01864naa a2200205 a 4500
001 1124373
005 2015-09-08
008 2015 bl uuuu u00u1 u #d
100 1 $aCUNHA, C. M. S.
245 $aA SCAR marker for identifying susceptibility to Fusarium oxysporum f. sp. cubense in banana.$h[electronic resource]
260 $c2015
520 $aFusarium wilt (also known as Panama disease) caused by Fusarium oxysporum Schlechtend. f. sp. cubense (E.F. Smith) W.C. Snyder & H.N. Hansen (Foc) is an endemic disease in all banana (Musa spp. L.) producing areas. The use of resistant cultivars is the recommended method for the disease control. The development of resistant or tolerant cultivars is expensive and time-consuming since the results must be confirmed by many years of field evaluation. In this situation, DNA markers show a great potential to improve the efficiency and precision of conventional plant breeding. Thus, the objective of this work was to develop a SCAR marker able to discriminate between resistant and susceptible genotypes to Foc infection. Using genomic DNA from resistant or susceptible genotypes and 78 10-mer arbitrary primers, one RAPD band associated to susceptibility was selected and used to generate the SCAR marker SuscPD-F/SuscPD-R. This marker was validated on 28 banana cultivars which were resistant or susceptible to infection by Foc. The results indicated a high degree of specificity of the marker, which was able to discriminate between the two contrasting groups (resistant or susceptible). The discriminatory power of the new marker was 93%.
653 $amolecular marker
653 $aPanama disease
700 1 $aHINZ, R. H.
700 1 $aPEREIRA, A.
700 1 $aTCACENCO, F. A.
700 1 $aPAULINO, E. C.
700 1 $aSTADNIK, M. J.
773 $tScientia Horticulturae, British Columbia$gv. 191, p. 108-112, 2015.
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Epagri-Sede (Epagri-Sede) |
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