Data/hora: 20/05/2024 14:35:30

Biblioteca(s): | Epagri-Sede.
Tipo da produção científica: | Resumo em Anais de Congresso
Circulação/Nível: | -- - --
Data corrente: | 07/04/2011
Data da última atualização: | 07/04/2011
Autoria: | VIEIRA NETO, J.
Afiliação: | Epagri
Título: | Genetic structure and characterization of olive germplasm using microsatellite molecular markers.
Ano de publicação: | 2010
Fonte/Imprenta: | In: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá. Resumos... Ribeirão Preto: SBG, 2010. p. 64-64.
ISBN: | 978-85-89109-06-2
Idioma: | Inglês

Conteúdo:

In Brazil there are still many uncertainties about names of olive varieties (Olea europeae L.), since it can be found different names for the same genotype (synonymy) or the same names for different genotypes (homonyms). Faced with this reality and the limitations of morphological markers in the identification of olive genotypes, this study aimed characterizing and studying the genetic diversity and structure of 60 olive accessions. This constitutes an important tool in the certification process of this specie, in addition to support the genitor selection in Brazilian breeding programs. Tissue samples for DNA isolation were harvested from adult plants in EPAMIG germplasm banks located in Experimental Farms of Caldas and Maria da Fé, MG, Brazil. DNA was extracted by CTAB method described by SAGHAI-Marrof et al. (1984) with minor modifications. For genetic analysis, we used four pairs of primers previously reported for microsatellite studies in O. europeae (GAPU 101, GAPU 11 e 17, UDO 99-009, and UDO99-019). The four markers used allowed differentiation of most of the 60 olive accessions. We identified 22 alleles with an average of 5.5 alleles per locus, confirming the efficiency of the markers for the detection of genetic polymorphisms. The values of polymorphic information content (PIC) were GAPU 101 (0.8121), GAPU 11 e 17 (0.6959), UDO 99-009 (0.5149) and UDO99-019 (0.1860). Considering the four loci analyzed, the probability of identity has reached a relatively low value (0.001), demonstrating the high efficiency of these loci for genetic differentiation of varieties. An unrooted cladogram was generated by the index of dissimilarity CS Chord (1967), using the clustering method Neighbor Joining Tree. These results showed the broad span of divergence time among the genotypes used in
this work. In the analysis of principal coordinates, two distinct groups were formed. However, the Bayesian analysis, performed with the Structure 2.3.3 software, has structured the 60 genotypes in three distinct groups. Hence, the SSR markers used were efficient for characterization and detection of genetic structure in these germplasm banks.

Palavras-Chave: | Diversidade genética; DNA; Fingerprinting; Genetic Diversity; Genetic Structure; Germoplasma; Germplasm evaluation; Oliva; SSR.
Categoria do assunto: | --
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_943.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (78168-1) - UPC - PL - -- -  -  -  - DIS