Data/hora: 26/06/2024 11:33:54

Biblioteca(s): | Epagri-Sede.
Data corrente: | 29/08/2017
Data da última atualização: | 29/08/2017
Autoria: | TANCREDO, K. R.; MARCHIORI, N. C.; PEREIRA, S. A.; MARTINS, M. L.
Título: | Minimum inhibitory concentration (MIC-test) of formalin for Dactylogyrus minutus Kulwièc, 1927 (Monogenea: Dactylogyridae): in vitro test.
Ano de publicação: | 2017
Fonte/Imprenta: | In: INTERNATIONAL SYMPOSIUM ON MONOGENEA, 8., 2017, Brno, Czech Republic. Abstracts... Brno, Czech Republic: ISM, 2017. p. 95
Idioma: | Inglês

Conteúdo:

The formaldehyde has been widely used in its diluted form as an alternative to malachite
green in the treatment of parasites and fungi. It has been used as anti-fungal and against
parasites, especially protozoans and monogeneans from the gills and body surface of fish
and immature amphibians like tadpoles. The minimum inhibitory concentration (MIC-test)
of formalin in vitro that controls the Dactylogyrus minutus Kulwièc, 1927 of Cyprinus
carpio var. koi was determined in this study. Samples of carp var. koi (1.73±0.25 g mean
weight and 4.7±0.46 cm total length) were obtained in September 2016 from a fish farm
located in Biguaçu, Santa Catarina, Brazil and transported live to Aquatic Organisms Health
Laboratory. Eight concentrations of formalin were tested: 50, 75, 100, 125, 150, 175, 200,
250 ppm. In addition, two controls were prepared, one with water from the recirculation
system and another with distilled water. Gill filaments from parasitized gill arches were
collected to obtain the adult specimens of D. minutus. Parasites were placed into 6-well flat
plates for cell culture in a volume of 3 ml per well, where each set of parasitized filaments
received a formalin concentration. Mortality observations were recorded every 5 minutes at
the highest concentrations or 10 minutes at the lowest concentrations using a
stereomicroscope. The parasites were considered dead, when the absence of movements to
the stimulus with needle was verified, as well as typical characteristics of mortality as the
wrinkling of the body. Data were submitted to analysis of variance (ANOVA) and Tukey's
test was used to compare means (p<0.05). The concentration of 200 ppm was the most lethal
to D. minutus, killing all the parasites in 16 minutes. However, for 250, 175, 125 and 75
ppm there was no statistical difference when compared to the two most lethal concentrations.
The concentration of 100 ppm took longer to act on all monogeneans, however the time (47
minutes) was not higher than the time normally recommended in fish treatment. As some
protocols indicate baths of at least 1 hour or even continuous baths, with this study, we could
guarantee the real efficacy of the formalin directly in the parasite by monitoring the time
and concentration.

Palavras-Chave: | Monogenea.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_39706.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
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