Data/hora: 26/06/2024 11:38:42

Biblioteca(s): | Epagri-Sede.
Data corrente: | 29/08/2017
Data da última atualização: | 29/08/2017
Autoria: | TANCREDO, K. R.; MARCHIORI, N. C.; MARTINS, M. L.
Título: | Morphometric and scanning electron microscopy of Dactylogyrus minutus Kulwièc, 1927 (Monogenea: Dactylogyridae) from the Cyprinus carpio var. koi.
Ano de publicação: | 2017
Fonte/Imprenta: | In: INTERNATIONAL SYMPOSIUM ON MONOGENEA, 8., 2017, Brno, Czech Republic. Abstracts... Brno, Czech Republic: ISM, 2017. p. 108
Idioma: | Inglês

Conteúdo:

Carp from the coloured koi variety is commonly used for both aquarism and earthen-pond
culture. However, its culture has been affected by dactylogyrid species, causing massive
mortality for more than four species of Dactylogyrus genus in the same host. In the present
study, Dactylogyrus minutus specimens living on the gills of Cyprinus carpio were
identified using light, confocal and scanning electron microscopy to characterize its
morphological and morphometrical characters by the first time in Brazil. Samples of carp
var. koi (weight of 1.73±0.25 g; total length of 4.7±0.46 cm) were obtained in September
2016 from a fish farm located in Biguaçu, Santa Catarina, Southern Brazil and transported
live to Aquatic Organisms Health Laboratory. After acclimatization period, thirty fish were
anesthetized in clove oil (75 mg/L) and were examined for the infestation intensity of
parasites on the gills using a stereomicroscope. Specimens of Dactylogyrus were isolated
and fixed in 70% ethanol for morphological identification, 10% formalin for confocal
microscopy and 2.5% phosphate-buffered glutaraldehyde for scanning electron microscopy.
The monogeneans were identified as Dactylogyrus minutus Kulwièc, 1927 (P 75%, MII
3.55±2.91, MA 2.66±2.95).In general, the measurements (&#956;m) of 20-Hoyer¿s mounted
specimens were close to those described by Kulwièc (1927): body 196-247 (216) long and
46-63 (53) wide at midbody, anchor total length 39-44 (42) long, with base length 33-37
(35) and point length 13-15 (14), anchor inner root 12-14 (13) long, outer root 5-6 (6) long.
One single bar 28-32 (30) long, 14 marginal hooks 20-23 (22) long, male copulatory organ
(MCO) 29-38 (34) total length. Oval egg 59-60 (60) long, 40-41 (40) total wide with one
small appendage 5-6 (6). A pair of long anchors supported by a single bar longer rounded in
the extremities at the dorsal side. In images obtained using confocal microscopy the shape
of the bar was improved, evidencing the point in the extremities with a slight groove. The
cirrus is a thin long tube which tapers anteriorly. There are two MCO accessory piece, one
has a side branch and diverges into two points, and has approximately the same size as cirrus.
Another piece is shorter originating at the opposite side. The vagina opens on the right side
of the body anteriorly to the middle of the body, as shown by scanning electron microscopy.
There was a previous report of D. minutus from Brazil in the same host but no morphological
data were related. It can be considered that this study supports all the previous
characterization, not only by the details of the haptor but also by the dorsal bar observation
in a confocal microscopy and scanning electron microscopy. In fact, using these techniques
the vaginal pore and specimens attached to the gills were observed. Morphometric
measurements are given for the first time from koi carp collected in a fish farm from Brazil,
which is important to highlight in case of a cosmopolitan fish parasite species.

Palavras-Chave: | Monogenea.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_39704.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (101581-1) - UPC - PL - DD -  -  -  - DIS