Data/hora: 23/05/2024 17:17:08

Biblioteca(s): | Epagri-Sede.
Data corrente: | 19/01/2015
Data da última atualização: | 19/01/2015
Autoria: | VIEIRA, R. L.; SILVA, A. L.; ZAFFARI, G. R.; STEINMACHER, D. A.; FRAGA, H. P. F.; GUERRA, M. P.
Título: | Efficient elimination of complex from garlic (allium sativum L.) by cryoterapy of shoot tips
Ano de publicação: | 2014
Fonte/Imprenta: | Acta Physiologiae Plantarum, Kraków, v. 37, n. 1733, p. 01-11, 2014.
Idioma: | Inglês

Conteúdo:

Vegetative propagation of plants, such as garlic (Allium sativum L.), is known to facilitate the transmission of several virus species throughout the plant cycles. This process favors the onset of complex diseases by accumulation of different species in the same plant, resulting in decreased productivity and production quality. Studies have reported the use of cryotherapy of shoot tips, or
meristematic clusters, as an efficient tool for obtaining virus-free plants. This study aimed to evaluate the ability of cryotherapy to eradicate virus complex in garlic plants. Bulbils naturally infected with Onion Yellow Dwarf Virus (OYDV), Leek Yellow Strip Virus (LYSV) and Garlic Common Latent Virus (GCLV) were employed as explants for different virus-cleaning treatments tested. Dot-ELISA
and RT-PCR analysis were used to demonstrate the presence/absence of virus complex, and histological analysis was also performed to confirm these results. Five days after cryotherapy, structural analysis revealed that cooling had caused cell damage, as indicated by the increased vacuolization of cells after cryotherapy, as well as slight plasmolysis after thermotherapy. Immunolocalization analysis indicated the subcellular distribution of OYDV in garlic shoot tips in association with the development of plasmodesmata, while no OYDV was detected in the first cell layers of the meristematic dome. Cryotherapy successfully removed virus complex, resulting in virus-free plants with enhanced efficiency, compared to conventional meristem culture-based techniques. Moreover, the synergistic effects of cryotherapy and thermotherapy resulted in a 40 % survival rate of shoot tips and the regeneration of 90, 100 and 80 % OYDV-, LYSV- and GCLV-free plants, respectively.

Palavras-Chave: | Alho; Cryopreservation; In vitro culture; Meristem culture; Shoot tips; Thermotherapy.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_35353.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
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