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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
04/10/2022 |
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Data da última atualização: |
04/10/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, M. C. C. R.; SANTOS, F.; FRANDOLOSO, V.; BRANCO, F. A.; BRUGNARA, E. C.; MEES, A. |
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Título: |
First report of 'Candidatus Liberibacter asiaticus' associated with three symptomatic 'Ponkan' citrus tree in Xanxerê, Santa Catarina. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: WORKSHOP BRASILEIRO DE EPIDEMIOLOGIA DE DOENÇAS DE PLANTAS, 6., 2022, Chapecó. Resumos... Brasília: Sociedade Brasileira de Fitopatologia, 2022. p. 35 |
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Idioma: |
Inglês |
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Conteúdo: |
Huanglongbing (HLB) is considered the most threatening disease of citrus. Etiological HLB agents are the Gram-negative bacteria in the Rhizobiaceae family, ?Candidatus Liberibacter asiaticus?, ?Ca. L. americanus? and ?Ca. L. africanus?. ?Ca L. asiaticus? is the main HLB bacteria with occurrence in Brazil, which transmission is aided by the Asian-citrus-psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae). The Brazilian South state Santa Catarina does not figure among the main citrus producers States in Brazil, but the orchards are mainly cultivated in small farms, which brings up Citrus spp. as an important agronomic alternative for family farmers. During an inspection service demanded by the Department of Plant Health and Agricultural Inputs of the Secretariat of Animal and Plant Health and Inspection (SDA) of the Ministry of Agriculture, Livestock and Food Supply (MAPA), regulated by a recent released ministerial order (Portaria 317, 21 may 2021), a suspect plant was spotted in a productive orchard in Xanxerê, Santa Catarina (geographical coordinates: -26.8774415, -52.406414). The branches of the mandarin plant (Citrus reticulata Blanco cv. Ponkan) had been already severely pruned by the grower few weeks before, as he noticed an unusual appearance and a decrease of production. When visited by the plant health officer, it was already emitting a new flush, with the typical appearing of blotchy mottle leaves, with downwards curvature and thickening of veins. A leaf was detached from this plant as a sample and addressed to a laboratory for molecular diagnosis. The laboratory carried out the protocol described in the Bulletin EPPO (44:376, 2014) for HLB pathogens detection, which involved the use of primer HLBr combined with primers HLBas, HLBam or HLBaf in a qPCR diagnostic protocol for ?Ca. L. asiaticus?, ?Ca. L. americanus? and ?Ca. L. africanus?, respectively, using HLBp as a FAM probe. To confirm the positive qPCR result for ?Ca. L. asiaticus?, a conventional multiplex PCR was performed with the forward primers OI1 and OA1 combined with reverse primer OI2c to detect ?Ca. L. asiaticus? and ?Ca. L. americanus?. A 1160 bp band was produced and the amplicon was sequenced for further in silico distinction using Xbal digestion of the amplicon. After ?Ca. L. asiaticus? detection in that plant, other plants in the very same orchard were inspected and surveyed for molecular diagnosis. HLB bacteria was also detected in other two ?Ponkan? tree that was displaying HLB symptoms as described above and producing lopsided fruit, with seed abortion and stain of the vascular columella. A contingence plan was adopted by the Plant Health Agency of Santa Catarina State (CIDASC), in which the infected plant with HLB bacteria has been eliminated by the grower. In addition, yellow sticky traps were installed within the orchard in Xanxerê in order to verify the presence of D. citri. Also, the orchards within a radio of 4 km are under inspection. The Plant Protection Agency is currently tracking back the origin of the plants in order to find out whether the diseased plants were introduced via nursery plant or the pathogen was transmitted via resident insect vector. MenosHuanglongbing (HLB) is considered the most threatening disease of citrus. Etiological HLB agents are the Gram-negative bacteria in the Rhizobiaceae family, ?Candidatus Liberibacter asiaticus?, ?Ca. L. americanus? and ?Ca. L. africanus?. ?Ca L. asiaticus? is the main HLB bacteria with occurrence in Brazil, which transmission is aided by the Asian-citrus-psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae). The Brazilian South state Santa Catarina does not figure among the main citrus producers States in Brazil, but the orchards are mainly cultivated in small farms, which brings up Citrus spp. as an important agronomic alternative for family farmers. During an inspection service demanded by the Department of Plant Health and Agricultural Inputs of the Secretariat of Animal and Plant Health and Inspection (SDA) of the Ministry of Agriculture, Livestock and Food Supply (MAPA), regulated by a recent released ministerial order (Portaria 317, 21 may 2021), a suspect plant was spotted in a productive orchard in Xanxerê, Santa Catarina (geographical coordinates: -26.8774415, -52.406414). The branches of the mandarin plant (Citrus reticulata Blanco cv. Ponkan) had been already severely pruned by the grower few weeks before, as he noticed an unusual appearance and a decrease of production. When visited by the plant health officer, it was already emitting a new flush, with the typical appearing of blotchy mottle leaves, with downwards curvature and thickening of veins. A leaf was det... Mostrar Tudo |
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Thesagro: |
Doença quarentenária; erradicação; liberibacter; transmissão por vetor. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 04030naa a2200229 a 4500
001 1132481
005 2022-10-04
008 2022 bl uuuu u00u1 u #d
100 1 $aSILVA, M. C. C. R.
245 $aFirst report of 'Candidatus Liberibacter asiaticus' associated with three symptomatic 'Ponkan' citrus tree in Xanxerê, Santa Catarina.$h[electronic resource]
260 $c2022
520 $aHuanglongbing (HLB) is considered the most threatening disease of citrus. Etiological HLB agents are the Gram-negative bacteria in the Rhizobiaceae family, ?Candidatus Liberibacter asiaticus?, ?Ca. L. americanus? and ?Ca. L. africanus?. ?Ca L. asiaticus? is the main HLB bacteria with occurrence in Brazil, which transmission is aided by the Asian-citrus-psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae). The Brazilian South state Santa Catarina does not figure among the main citrus producers States in Brazil, but the orchards are mainly cultivated in small farms, which brings up Citrus spp. as an important agronomic alternative for family farmers. During an inspection service demanded by the Department of Plant Health and Agricultural Inputs of the Secretariat of Animal and Plant Health and Inspection (SDA) of the Ministry of Agriculture, Livestock and Food Supply (MAPA), regulated by a recent released ministerial order (Portaria 317, 21 may 2021), a suspect plant was spotted in a productive orchard in Xanxerê, Santa Catarina (geographical coordinates: -26.8774415, -52.406414). The branches of the mandarin plant (Citrus reticulata Blanco cv. Ponkan) had been already severely pruned by the grower few weeks before, as he noticed an unusual appearance and a decrease of production. When visited by the plant health officer, it was already emitting a new flush, with the typical appearing of blotchy mottle leaves, with downwards curvature and thickening of veins. A leaf was detached from this plant as a sample and addressed to a laboratory for molecular diagnosis. The laboratory carried out the protocol described in the Bulletin EPPO (44:376, 2014) for HLB pathogens detection, which involved the use of primer HLBr combined with primers HLBas, HLBam or HLBaf in a qPCR diagnostic protocol for ?Ca. L. asiaticus?, ?Ca. L. americanus? and ?Ca. L. africanus?, respectively, using HLBp as a FAM probe. To confirm the positive qPCR result for ?Ca. L. asiaticus?, a conventional multiplex PCR was performed with the forward primers OI1 and OA1 combined with reverse primer OI2c to detect ?Ca. L. asiaticus? and ?Ca. L. americanus?. A 1160 bp band was produced and the amplicon was sequenced for further in silico distinction using Xbal digestion of the amplicon. After ?Ca. L. asiaticus? detection in that plant, other plants in the very same orchard were inspected and surveyed for molecular diagnosis. HLB bacteria was also detected in other two ?Ponkan? tree that was displaying HLB symptoms as described above and producing lopsided fruit, with seed abortion and stain of the vascular columella. A contingence plan was adopted by the Plant Health Agency of Santa Catarina State (CIDASC), in which the infected plant with HLB bacteria has been eliminated by the grower. In addition, yellow sticky traps were installed within the orchard in Xanxerê in order to verify the presence of D. citri. Also, the orchards within a radio of 4 km are under inspection. The Plant Protection Agency is currently tracking back the origin of the plants in order to find out whether the diseased plants were introduced via nursery plant or the pathogen was transmitted via resident insect vector.
650 $aDoença quarentenária
650 $aerradicação
650 $aliberibacter
650 $atransmissão por vetor
700 1 $aSANTOS, F.
700 1 $aFRANDOLOSO, V.
700 1 $aBRANCO, F. A.
700 1 $aBRUGNARA, E. C.
700 1 $aMEES, A.
773 $tIn: WORKSHOP BRASILEIRO DE EPIDEMIOLOGIA DE DOENÇAS DE PLANTAS, 6., 2022, Chapecó. Resumos... Brasília: Sociedade Brasileira de Fitopatologia, 2022. p. 35
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