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| Registros recuperados : 168 | |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
17/01/2020 |
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Data da última atualização: |
17/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - B |
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Autoria: |
YAMASHITA, M. M.; FERRAREZI, J. V.; PEREIRA, G. V.; BANDEIRA JÚNIOR, G.; SILVA, B. C.; PEREIRA, S. A.; MARTINS, M. L.; MOURIÑO, J. L. P. |
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Título: |
Autochthonous vs allochthonous probiotic strains to Rhamdia quelen. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Microbial Pathogenesis, Amsterdã, Países Baixos, n. 139, p. 103897, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to obtain an autochthonous probiotic candidate strain from the silver catfish (Rhamdia quelen) intestinal tract, comparing its in vivo performance with an allochthonous probiotic isolated from another fish, Nile tilapia (Oreochromis niloticus), in a growth performance assay. The study was divided in two parts: in vitro and in vivo assay followed by challenge with A. hydrophila. In the in vitro assay, the species-specific isolated strain Lactococcus lactis presented characteristics such as: absence of hemolysis, antagonism to bacterial pathogens isolated from freshwater fish, and considerable speed of duplication. In the in vivo trial, both fish supplemented with autochthonous or allochthonous strains presented an increase the final concentration of lactic acid bacteria in the intestinal tract of the fish after 60 days of dietary supplementation reaching con- −1 −1 7 7 centrations of 1 × 10 CFU g and 4 × 10 UFC.g , respectively. In addition, the autochthonous strain increased the mean corpuscular hemoglobin (MCH) of the treated animals, but no significant differences were observed in the other hemato-immunological and zootechnical parameters between treatments. After challenge with Aeromonas hydrophila, only animals that received autochthonous probiotic supplementation showed an increase in the serum total immunoglobulin concentration, but not enough to observe a significant difference in the survival rate between the treatments. Dietary supplementation of the probiotic allochthonous strain did not demonstrate any effects superior to those of the isolated autochthonous strain. Although the autochthonous strain did not present significant improvements in the other parameters evaluated in this study, it was able to inhibit bacterial pathogens in vitro, to increase the final concentration of LAB's and the amount of im- munoglobulin after experimental challenge, demonstrating probiotic potential. This study demonstrated for the first time the isolation and in vivo use of an autochthonous probiotic strain isolated from silver catfish, as well as its comparative evaluation with the performance of allochthonous probiotic. MenosThe aim of this study was to obtain an autochthonous probiotic candidate strain from the silver catfish (Rhamdia quelen) intestinal tract, comparing its in vivo performance with an allochthonous probiotic isolated from another fish, Nile tilapia (Oreochromis niloticus), in a growth performance assay. The study was divided in two parts: in vitro and in vivo assay followed by challenge with A. hydrophila. In the in vitro assay, the species-specific isolated strain Lactococcus lactis presented characteristics such as: absence of hemolysis, antagonism to bacterial pathogens isolated from freshwater fish, and considerable speed of duplication. In the in vivo trial, both fish supplemented with autochthonous or allochthonous strains presented an increase the final concentration of lactic acid bacteria in the intestinal tract of the fish after 60 days of dietary supplementation reaching con- −1 −1 7 7 centrations of 1 × 10 CFU g and 4 × 10 UFC.g , respectively. In addition, the autochthonous strain increased the mean corpuscular hemoglobin (MCH) of the treated animals, but no significant differences were observed in the other hemato-immunological and zootechnical parameters between treatments. After challenge with Aeromonas hydrophila, only animals that received autochthonous probiotic supplementation showed an increase in the serum total immunoglobulin concentration, but not enough to observe a significant di&#... Mostrar Tudo |
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Palavras-Chave: |
Disease resistance; Immunoglobulin; Lactic acid bacteria; Lactobacillus plantarum; Lactococcus lactis; sh; Silver catfi. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03137naa a2200289 a 4500
001 1129387
005 2020-01-17
008 2020 bl uuuu u00u1 u #d
100 1 $aYAMASHITA, M. M.
245 $aAutochthonous vs allochthonous probiotic strains to Rhamdia quelen.$h[electronic resource]
260 $c2020
520 $aThe aim of this study was to obtain an autochthonous probiotic candidate strain from the silver catfish (Rhamdia quelen) intestinal tract, comparing its in vivo performance with an allochthonous probiotic isolated from another fish, Nile tilapia (Oreochromis niloticus), in a growth performance assay. The study was divided in two parts: in vitro and in vivo assay followed by challenge with A. hydrophila. In the in vitro assay, the species-specific isolated strain Lactococcus lactis presented characteristics such as: absence of hemolysis, antagonism to bacterial pathogens isolated from freshwater fish, and considerable speed of duplication. In the in vivo trial, both fish supplemented with autochthonous or allochthonous strains presented an increase the final concentration of lactic acid bacteria in the intestinal tract of the fish after 60 days of dietary supplementation reaching con- −1 −1 7 7 centrations of 1 × 10 CFU g and 4 × 10 UFC.g , respectively. In addition, the autochthonous strain increased the mean corpuscular hemoglobin (MCH) of the treated animals, but no significant differences were observed in the other hemato-immunological and zootechnical parameters between treatments. After challenge with Aeromonas hydrophila, only animals that received autochthonous probiotic supplementation showed an increase in the serum total immunoglobulin concentration, but not enough to observe a significant difference in the survival rate between the treatments. Dietary supplementation of the probiotic allochthonous strain did not demonstrate any effects superior to those of the isolated autochthonous strain. Although the autochthonous strain did not present significant improvements in the other parameters evaluated in this study, it was able to inhibit bacterial pathogens in vitro, to increase the final concentration of LAB's and the amount of im- munoglobulin after experimental challenge, demonstrating probiotic potential. This study demonstrated for the first time the isolation and in vivo use of an autochthonous probiotic strain isolated from silver catfish, as well as its comparative evaluation with the performance of allochthonous probiotic.
653 $aDisease resistance
653 $aImmunoglobulin
653 $aLactic acid bacteria
653 $aLactobacillus plantarum
653 $aLactococcus lactis
653 $ash
653 $aSilver catfi
700 1 $aFERRAREZI, J. V.
700 1 $aPEREIRA, G. V.
700 1 $aBANDEIRA JÚNIOR, G.
700 1 $aSILVA, B. C.
700 1 $aPEREIRA, S. A.
700 1 $aMARTINS, M. L.
700 1 $aMOURIÑO, J. L. P.
773 $tMicrobial Pathogenesis, Amsterdã, Países Baixos$gn. 139, p. 103897, 2020.
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