Data/hora: 16/06/2024 08:36:19

Biblioteca(s): | Epagri-Sede.
Tipo da produção científica: | Resumo em Anais de Congresso
Circulação/Nível: | -- - --
Data corrente: | 10/08/2011
Data da última atualização: | 10/08/2011
Autoria: | GERGER, R. P. C.; FORELL, F.; MEZZALIRA, J. C.; ZAGO, F. C.; VIEIRA, F. K.; MACHADO JÚNIOR, J.; OHLWEILER, L. U.; RAUBER, L. P.; RODRIGUES, J. L. R.; AMBRÓSIO, C. E.; MIGLINO, M. A.; MEZZALIRA, A.; BERTOLINI, M.
Afiliação: | Epagri
Título: | Effect of Fusion-Activation Interval and Embryo Aggregation on In Vitro and In Vivo Development of Cloned Bovine Embryos Produced by Handmade Cloning.
Ano de publicação: | 2010
Fonte/Imprenta: | In: ANNUAL CONFERENCE OF IETS, 2010, Córdoba, Argentina. Proceedings... Sydney, Austrália: Csiro, 2010. p. 185-186.
Idioma: | Português
Conteúdo: | Despite the rather success of cloning by somatic cell nuclear transfer (SCNT), the efficiency in development to term remains low, with a high rate of losses occurring throughout pregnancy due to faulty reprogramming and conceptus abnormalities. As the ideal fusion-activation interval for optimal nuclear reprogramming after cloning is still ill-defined, the aim of this study was to determine the effect of two distinct fusion-activation intervals and embryo aggregation on in vitro and in vivo development of clone bovine embryos. Increasing the fusion-activation interval to 4 h decreased cleavage but not blastocyst rates in 1 x 100% embryos. Blastocyst rates were lower in 1 x 100% embryos activated 2 h post-fusion. In general, cleavage and blastocyst rates for 2 x 100% embryos were higher than for 1 x 100% embryos (91.5% and 46.0% vs. 74.4% and 31.3%, respectively), irrespective of the activation time. Blastocyst rates for 4 hpf-activated embryos, based on cleavage, were higher than for 2 hpf-activated embryos (50.3% vs. 38.3%, respectively), despite the IVC scheme. Despite differences in in vitro development, pregnancy rates and conceptus development during the first two trimesters of pregnancy were similar between groups. A longer fusion-activation interval (4 hpf) or embryo aggregation scheme (2 x 100%) increased blastocyst yield but did not improve in vivo development nor pregnancy maintenance following the transfer to female recipients in cattle.
Palavras-Chave: | Bovine; Clone; Embryos; Handmade; in vitro; in vivo.
Categoria do assunto: | --
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_6262.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (82114-1) - UPC - PL - -- -  -  -  - DIS