Data/hora: 08/06/2024 14:57:18

Biblioteca(s): 	Epagri-Sede.	
Tipo da produção científica: 	Resumo em Anais de Congresso	
Data corrente: 	06/09/2019	
Data da última atualização: 	06/09/2019	
Autoria: 	ARAUJO, L.; CARDOZA, Y. F.; DUARTE, V.; MORAES, M. G.	
Título: 	Symptoms of bacterial shoot blight associated with Pseudomonas syringae pv. syringae in apple trees in Brazil.	
Ano de publicação: 	2019	
Fonte/Imprenta: 	In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife, PE. Resumos... Recife, PE: Sociedade Brasileira de Fitopatologia, 2019. p. 92	
Idioma: 	Inglês	
Conteúdo: 	Diseases of fruit trees caused by Pseudomonas syringae are a major concern in fruit producing areas worldwide. In
Brazil, apple plants showing bacterial symptoms were observed in a commercial orchard in São Joaquim, SC, during
the summer of 2016. Thus, the objective of the present study was to identify the causal organism of those
symptoms. Isolation from the affected tree samples was performed on King?s B (KB) medium. After 48 hours at 28 °C,
most of bacterial isolates formed round, shiny, gray-white colonies producing green fluorescent pigment. The Gramnegative
bacterium belongs to the group Ia (LOPAT +???+). Detached leaves of cv. Gala and immature fruits of cv.
Braeburn, Fuji, Kinkas, and Pink Lady of apple, inoculated with a bacterial suspension, showed intense necrosis and
decay, respectively, four days after inoculation (d.a.i.). Similarly, 1.5-year-old apple plants cv. Gala with Marubakaido
+ M.9 interstock, grown in plastic pots with 1 kg of substrate, kept in greenhouse, presented wilting, leaf blight, dark
xylem tissue visible along the branch length, and red-rust bacterial ooze, 30 d.a.i. The bacterium with the same
characteristics was readily re-isolated from the different infected tissues. LOPAT tests indicated the isolates
belonged to P. syringae pv. syringae. The taxonomic positioning of isolate was determined using Genome-to-
Genome Distance Calculator, by in silico DNA-DNA hybridization and showed 93.9% similarity with P. syringae strain
31R1.One isolate was deposited in the IBSBF Culture Collection (Instituto Biológico, SP, Brazil) and cataloged as
#IBSBF 3261. Genome sequence was deposited in the Genbank, accession number: QOSP00000000. To our
knowledge this is the first report of P. syringae pv. syringae causing bacterial canker in commercial orchards in Brazil.	
Palavras-Chave: 	bacterial canker; bacterial ooze; internal necrosis.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
URL: 	http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_43229.pdf	
Registro original: 	Epagri-Sede (Epagri-Sede)	
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