Data/hora: 08/06/2024 14:27:57

Biblioteca(s): | Epagri-Sede.
Tipo da produção científica: | Resumo em Anais de Congresso
Data corrente: | 11/09/2018
Data da última atualização: | 11/09/2018
Autoria: | CARDOZA, Y. F.; ARAUJO, L.; DUARTE, V.; MORAES, M. G.
Título: | Identification of Pectobacterium carotovorum as the causal agent of bacterial canker on Pyrus communis L in Brazil.
Ano de publicação: | 2018
Fonte/Imprenta: | In: INTERNATIONAL CONGRESS OF PLANT PATHOLOGY, 11., 2018, Boston, USA. Abstracts... Minneapolis - Minnesota - USA: American Phytopathological Society, 2018. p. 984
Idioma: | Inglês

Conteúdo:

In June 2016, bacterial canker-like symptoms were observed on Pyrus communis L cv. Yali in one commercial orchard
in Monte Castelo, SC. Eight-hundred of 1.200 plants were lost by death and, finally, tree eradication. In affected trees,
the disease manifested as cankers with exudates on trunks and twigs, and the stems turned brown and slowly died.
Bacteria were isolated on King B medium from infected tissues. Biochemical tests including Biolog® GEN III were
conducted and some isolates were selected for whole genome sequencing throught Ion Torrent? platform. PCR with
Y1/Y2 and PcaF/PcaR primers, specific for P. carotovorum and P. carotovorum subsp. actinidiae respectively were
also carried out. A total of 15 bacterial colonies were isolated from infected tissues. All were gram-negative,
facultatively anaerobic, rod-shaped and exhibited a strong pectolitic activity in potato and carrot disks. Biolog assay
showed that the isolates were homogeneus, and three of them were selected for further tests. The organism with the
highest genome and proteome identity (99.8%) with the isolates was Pectobacterium carotovorum subsp. actinidiae. A
product of the expected size was obtained in both especific PCR. Inoculation of pear tree branches with 24h old
colonies showed that the bacterium was able to colonize the plant tissues. More tests, including kiwifruit inoculation,
are in progress to fulfill Koch?s postulates. To our knowledge, this is the first report of P. carotovorum causing pear
bacterial canker-like symptoms in Brazil. Identification and monitoring of this pathogen is very important due to large
economic losses that can cause

Palavras-Chave: | apple diseases; bacterial diseases; malus domestica.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_41246.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (102693-1) - UPC - PL - DD -  -  -  - DIS