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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
27/10/2023 |
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Data da última atualização: |
27/10/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MANFIO, C. E.; MARO, L. A. C.; PASSOS, J. F. M.; COSTA, M. D. |
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Título: |
DIAGNÓSTICO DE DOENÇAS UTILIZANDO QPCR EM MUDAS CÍTRICAS REGENERADAS APÓS LIMPEZA CLONAL. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8., 2023, Florianópolis, SC. Resumos... São Paulo, SP: Sociedade Brasileira de Genética, 2023. p. 52 |
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Idioma: |
Inglês |
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Conteúdo: |
The citrus seedlings sector in Santa Catarina has experienced considerable expansion in recent years with an
increase in the number of seedlings produced and also in the number of families involved in the activity. The
lack of important diseases that commonly occur in other states of the federation could put the orchards in Santa
Catarina, the seedling producing region and also the experimental collections kept in the open by Epagri at
risk. In order for the activity to be profitable, whether as a fruit producer or seedling producer, it is essential
that the propagation materials used for the formation of seedlings are free of any pathogens. Therefore, the
diagnosis of diseases is extremely important, and clonal cleaning by micrografting is a primordial tool to ensure
the absence of viruses that are highly harmful to the exploitation of this crop. In view of the above, the objective
of this work was to certify the health of citrus seedlings regenerated after clonal cleaning. To obtain the
seedlings, buds from parent plants of the SCS454 Catarina, SCS455 Reinaldo, SCS 456 Sigmar, SCS 457
Souza and SCS458 Osvino cultivars were used. The qPCR technique was used to verify the micrografting
efficiency. Twenty-one seedlings were regenerated in vitro and had their RNA extracted using the Trizol
protocol. The qPCR reactions for analysis of CEVd (Citrus exocortis viroid) and HSV (Hop stunt viroid)
contained 1 μL of cDNA, 10 μL of master Mix (Sybr Green), 0.35 μM of primers, and water to make up the
final volume of 20 μL, and for the qPCR reactions for psorosis, the protocol by Francesco et al., 2014 was
used. Of the twenty-one seedlings, seventeen showed neither the presence of viroids nor Psorosis. The four
seedlings that were positive did not show both diseases together. Although the four seedlings were not
completely cleaned, they showed only one disease. Important results, since they confirm the efficiency of the
clonal cleaning technique through micrografting in obtaining basic material with phytosanitary quality. The
phytosanitary quality of the citrus seedling produced is a basic requirement for the frank development of the
activity and to place the seedling in Santa Catarina on a differentiated level, precisely because it does not occur
important diseases that are already diagnosed in other states of the federation. In this way, it will be possible
to transfer the benefits of biotechnology to the productive sector for the use of healthy propagation material by
nurserymen in the formation of seedlings and, consequently, contributing to the formation of long-lived
orchards. In this sense, the clonal cleaning technique by means of micrografting was efficient for obtaining
material free of viroids and psorisis. MenosThe citrus seedlings sector in Santa Catarina has experienced considerable expansion in recent years with an
increase in the number of seedlings produced and also in the number of families involved in the activity. The
lack of important diseases that commonly occur in other states of the federation could put the orchards in Santa
Catarina, the seedling producing region and also the experimental collections kept in the open by Epagri at
risk. In order for the activity to be profitable, whether as a fruit producer or seedling producer, it is essential
that the propagation materials used for the formation of seedlings are free of any pathogens. Therefore, the
diagnosis of diseases is extremely important, and clonal cleaning by micrografting is a primordial tool to ensure
the absence of viruses that are highly harmful to the exploitation of this crop. In view of the above, the objective
of this work was to certify the health of citrus seedlings regenerated after clonal cleaning. To obtain the
seedlings, buds from parent plants of the SCS454 Catarina, SCS455 Reinaldo, SCS 456 Sigmar, SCS 457
Souza and SCS458 Osvino cultivars were used. The qPCR technique was used to verify the micrografting
efficiency. Twenty-one seedlings were regenerated in vitro and had their RNA extracted using the Trizol
protocol. The qPCR reactions for analysis of CEVd (Citrus exocortis viroid) and HSV (Hop stunt viroid)
contained 1 μL of cDNA, 10 μL of master Mix (Sybr Green), 0.35 μM of pri... Mostrar Tudo |
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Thesagro: |
Key-words: Citrus spp; micrografting; real-time; seedling production. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03501naa a2200205 a 4500
001 1133863
005 2023-10-27
008 2023 bl uuuu u00u1 u #d
100 1 $aMANFIO, C. E.
245 $aDIAGNÓSTICO DE DOENÇAS UTILIZANDO QPCR EM MUDAS CÍTRICAS REGENERADAS APÓS LIMPEZA CLONAL.$h[electronic resource]
260 $c2023
520 $aThe citrus seedlings sector in Santa Catarina has experienced considerable expansion in recent years with an increase in the number of seedlings produced and also in the number of families involved in the activity. The lack of important diseases that commonly occur in other states of the federation could put the orchards in Santa Catarina, the seedling producing region and also the experimental collections kept in the open by Epagri at risk. In order for the activity to be profitable, whether as a fruit producer or seedling producer, it is essential that the propagation materials used for the formation of seedlings are free of any pathogens. Therefore, the diagnosis of diseases is extremely important, and clonal cleaning by micrografting is a primordial tool to ensure the absence of viruses that are highly harmful to the exploitation of this crop. In view of the above, the objective of this work was to certify the health of citrus seedlings regenerated after clonal cleaning. To obtain the seedlings, buds from parent plants of the SCS454 Catarina, SCS455 Reinaldo, SCS 456 Sigmar, SCS 457 Souza and SCS458 Osvino cultivars were used. The qPCR technique was used to verify the micrografting efficiency. Twenty-one seedlings were regenerated in vitro and had their RNA extracted using the Trizol protocol. The qPCR reactions for analysis of CEVd (Citrus exocortis viroid) and HSV (Hop stunt viroid) contained 1 μL of cDNA, 10 μL of master Mix (Sybr Green), 0.35 μM of primers, and water to make up the final volume of 20 μL, and for the qPCR reactions for psorosis, the protocol by Francesco et al., 2014 was used. Of the twenty-one seedlings, seventeen showed neither the presence of viroids nor Psorosis. The four seedlings that were positive did not show both diseases together. Although the four seedlings were not completely cleaned, they showed only one disease. Important results, since they confirm the efficiency of the clonal cleaning technique through micrografting in obtaining basic material with phytosanitary quality. The phytosanitary quality of the citrus seedling produced is a basic requirement for the frank development of the activity and to place the seedling in Santa Catarina on a differentiated level, precisely because it does not occur important diseases that are already diagnosed in other states of the federation. In this way, it will be possible to transfer the benefits of biotechnology to the productive sector for the use of healthy propagation material by nurserymen in the formation of seedlings and, consequently, contributing to the formation of long-lived orchards. In this sense, the clonal cleaning technique by means of micrografting was efficient for obtaining material free of viroids and psorisis.
650 $aKey-words: Citrus spp
650 $amicrografting
650 $areal-time
650 $aseedling production
700 1 $aMARO, L. A. C.
700 1 $aPASSOS, J. F. M.
700 1 $aCOSTA, M. D.
773 $tIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8., 2023, Florianópolis, SC. Resumos... São Paulo, SP: Sociedade Brasileira de Genética, 2023. p. 52
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Registro original: |
Epagri-Sede (Epagri-Sede) |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
16/12/2016 |
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Data da última atualização: |
16/12/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - B |
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Autoria: |
ZANOTTO, E.; GUIMARÃES, R. A.; ZANOTTO, L. A. S.; MACHADO, J. C.; MELO, I. S.; PINHO, R. G. V.; PINTO, F. A. M. F.; FORTES, P. H. O. S.; MEDEIROS, H. N.; MEDEIROS, F. H. V. |
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Título: |
Effect of integrating fungicide and biocontrol foliar sprays on maize grain yield and fumonisin content. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
IOBC-WPRS Bulletin, Darmstadt, v. 117, p. 79-82, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
The adoption of biocontrol is low in maize production although its synergistic
action with fungicides on the grain content of fumonisin is reported. The objective of this
work was evaluate the potential effect of Bacillus sp. (BIOUFLA2) and Streptomyces
araujoniae (BIOUFLA1) combined or not with fungicide (azoxystrobin + ciproconazol) on
grain yield, Fusarium verticillioides, incidence and total fumonisin content. Maize plants
were sprayed two times, at the phenological stages V9 and R1 with water, BIOUFLA1,
BIOUFLA2, fungicide alone or in different combinations two by two. All plants were
inoculated with F. verticillioides. Upon harvest, treatments were evaluated for total yield,
F. verticillioides grain contamination (blotter test) and total fumonisin (B1 and B2) contents.
The treatment combination fungicide at V9-V10 combined to S. araujoniae at R1 resulted in
increased yield in three out of the four field trials, while all the other treatment combinations
resulted in increased yield in two out of the four trials. All treatment combinations less the
two sprays of fungicide reduced F. verticillioides incidence in grains compared to the water
control. None of the treatments contributed to the reduction on the fumonisin content
compared to the control but the two sprays of fungicides or S. araujoniae and the combination
S.araujoniae (V9-V10) + Fungicide (R1) resulted in higher mycotoxin contents compared to
the control. The use of the fungicide at V9 followed by S. araujoniae (R1) resulted in more
consistent yield increases, reduced grain contamination by F. verticillioides and did not
increase the fumonisin content compared to the control. MenosThe adoption of biocontrol is low in maize production although its synergistic
action with fungicides on the grain content of fumonisin is reported. The objective of this
work was evaluate the potential effect of Bacillus sp. (BIOUFLA2) and Streptomyces
araujoniae (BIOUFLA1) combined or not with fungicide (azoxystrobin + ciproconazol) on
grain yield, Fusarium verticillioides, incidence and total fumonisin content. Maize plants
were sprayed two times, at the phenological stages V9 and R1 with water, BIOUFLA1,
BIOUFLA2, fungicide alone or in different combinations two by two. All plants were
inoculated with F. verticillioides. Upon harvest, treatments were evaluated for total yield,
F. verticillioides grain contamination (blotter test) and total fumonisin (B1 and B2) contents.
The treatment combination fungicide at V9-V10 combined to S. araujoniae at R1 resulted in
increased yield in three out of the four field trials, while all the other treatment combinations
resulted in increased yield in two out of the four trials. All treatment combinations less the
two sprays of fungicide reduced F. verticillioides incidence in grains compared to the water
control. None of the treatments contributed to the reduction on the fumonisin content
compared to the control but the two sprays of fungicides or S. araujoniae and the combination
S.araujoniae (V9-V10) + Fungicide (R1) resulted in higher mycotoxin contents compared to
the control. The use of the fungicide at V9 followed by S. araujonia... Mostrar Tudo |
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Palavras-Chave: |
biological control; Fumonisins; integrated management. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02468naa a2200265 a 4500
001 1125886
005 2016-12-16
008 2016 bl uuuu u00u1 u #d
100 1 $aZANOTTO, E.
245 $aEffect of integrating fungicide and biocontrol foliar sprays on maize grain yield and fumonisin content.$h[electronic resource]
260 $c2016
520 $aThe adoption of biocontrol is low in maize production although its synergistic action with fungicides on the grain content of fumonisin is reported. The objective of this work was evaluate the potential effect of Bacillus sp. (BIOUFLA2) and Streptomyces araujoniae (BIOUFLA1) combined or not with fungicide (azoxystrobin + ciproconazol) on grain yield, Fusarium verticillioides, incidence and total fumonisin content. Maize plants were sprayed two times, at the phenological stages V9 and R1 with water, BIOUFLA1, BIOUFLA2, fungicide alone or in different combinations two by two. All plants were inoculated with F. verticillioides. Upon harvest, treatments were evaluated for total yield, F. verticillioides grain contamination (blotter test) and total fumonisin (B1 and B2) contents. The treatment combination fungicide at V9-V10 combined to S. araujoniae at R1 resulted in increased yield in three out of the four field trials, while all the other treatment combinations resulted in increased yield in two out of the four trials. All treatment combinations less the two sprays of fungicide reduced F. verticillioides incidence in grains compared to the water control. None of the treatments contributed to the reduction on the fumonisin content compared to the control but the two sprays of fungicides or S. araujoniae and the combination S.araujoniae (V9-V10) + Fungicide (R1) resulted in higher mycotoxin contents compared to the control. The use of the fungicide at V9 followed by S. araujoniae (R1) resulted in more consistent yield increases, reduced grain contamination by F. verticillioides and did not increase the fumonisin content compared to the control.
653 $abiological control
653 $aFumonisins
653 $aintegrated management
700 1 $aGUIMARÃES, R. A.
700 1 $aZANOTTO, L. A. S.
700 1 $aMACHADO, J. C.
700 1 $aMELO, I. S.
700 1 $aPINHO, R. G. V.
700 1 $aPINTO, F. A. M. F.
700 1 $aFORTES, P. H. O. S.
700 1 $aMEDEIROS, H. N.
700 1 $aMEDEIROS, F. H. V.
773 $tIOBC-WPRS Bulletin, Darmstadt$gv. 117, p. 79-82, 2016.
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