Data/hora: 06/06/2024 07:57:20

Biblioteca(s): | Epagri-Sede.
Tipo da produção científica: | Artigo em Anais de Congresso / Nota Técnica
Circulação/Nível: | -- - --
Data corrente: | 10/10/2011
Data da última atualização: | 10/10/2011
Autoria: | CASALI, R.; ZAGO, F. C.; FEDERLE, M.; DRUM, J. N.; SPONCHIADO, M.; KLEIN, N.; ZANETTI, M. S.; GAUDÊNCIO NETO, S.; MEZZALIRA, A.; COSTA, U. M.; VIEIRA, A. D.
Afiliação: | Epagri
Título: | Lyophilized seminal plasma improves the motility of frozen RAM semen and increases the cleavage in heterologous IVF.
Ano de publicação: | 2011
Fonte/Imprenta: | In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco, CE . [Proceedings...]. Porto Alegre, RS: UFRGS, 2011. p. 356.
Idioma: | Português
Conteúdo: | The addition of heterologous seminal plasma (HSP) to the freezing extender of ram semen improved post-thaw viability parameters, and might be an alternative to improve intra-cervical insemination and in vitro fertilization (IVF) with frozen ram semen. However, data regarding such conditions are still very scarce, especially for lyophilized plasma. The aim of this study was to evaluate the effect of addition of lyophilized bovine seminal plasma (LBSP) and equine (LESP) to the freezing extender of ram semen. The evaluations performed were: progressive motility (PM) after thaw (AT) and PM after percoll selection (PS), acrosome integrity (AI) after PS and cleavage rates after IVF with heterologous bovine oocytes. Seminal plasma was obtained from five bulls and five stallions collected with artificial vagina. The ejaculates were centrifuged (5000 rpm) and lyophilized with Christ Alpha 1-4 Freeze Dryer. The ram semen was collected with artificial vagina from four Texel rams, pooled and diluted in 1 +1 in glycerolated Tris-egg yolk without seminal plasma (Tris control, TC) or added of 600¥ìg/mL of LBSP or 600¥ìg/mL of LESP. The semen was then cooled at 0.3 ¡ÆC / min. until to 5 ¨¬C, stabilized for one hour, loaded in 0.25 mL straws in isothermal conditions and frozen at -80 ¡ÆC in LN2 vapor before store in cryogenic container at -196 ¡ÆC. The sperm viability was determined based on the PM-AT and PM-PS. The sperm were used for heterologous IVF (Garcia-Alvarez, 2009, Theriogenology, 71: 643-650), and to evaluate the acrosome reaction by FITC method (Sukardi Ani Sci Rep 1997, 46: 89-96). Data were analyzed by Student T test with significance level of 5%. There was no difference between the average rates of post thaw progressive motility PM-AT of PSLB (27.5%) and PSLE (37.5%) groups, but both were higher than TC group (10%), improving the viability of thawed semen by the addition of heterologous seminal plasma. The rates of PM-PS were similar in all groups (TC 40.0%, 56.7% LBSP, LESP 70.0%). There was no difference in the IAPP evaluation of different groups (CT 58.4%, 67.3% LBSP, LESP 63.3%). There was a significant increase in cleavage rate of LBSP (45.7%) and LESP (43.7%) groups when compared to TC group (32.6%). The increased cleavage rate observed in heterologous IVF demonstrates that the frozen semen added of LEAP or LBSP has a greater oocyte penetration ability, which suggests that they may be more efficient for sheep artificial insemination.
Palavras-Chave: | Cryopreservation; Heterologous seminal plasma; Lyophilization.
Categoria do assunto: | --
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_6394.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (84221-1) - UPC - PL - -- -  -  -  - DIS