Data/hora: 29/06/2024 07:58:04

Biblioteca(s): | Epagri-Sede.
Data corrente: | 15/04/2016
Data da última atualização: | 15/04/2016
Autoria: | MOURINO, J. L. P.; PEREIRA, G. V.; VIEIRA, F. N.; JATOBA, A.; USHIZIMA, T. T.; SILVA, B. C.; SEIFFERT, W. Q.; JESUS, G. F. A.; MARTINS, M. L.
Título: | Isolation of probiotic bacteria from the hybrid South Americancatfish Pseudoplatystoma reticulatum×Pseudoplatystoma corruscans (Siluriformes: Pimelodidae): A haematological approach.
Ano de publicação: | 2016
Fonte/Imprenta: | Aquaculture Reports, Filadélfia, EUA, v. 3, n. , p. 166-171, 2016.
Idioma: | Inglês
Conteúdo: | This study investigated bacterial strains with probiotic potential isolated from the middle portion of healthy hybrid surubim cat&#64257;sh foregut (Pseudoplatystoma reticulatum female× P. corruscans male). Twenty surubims weighing 1.5± 0.3 kg were used for bacterial isolation. In total, 41 strains of bacteria were selected in vitro. Ten strains had inhibition zones >10mm against Aeromonas hydrophila. Five of those strains presented inhibition zones > 9mm against other pathogenic bacteria and reached concentrations greater than 10 CFUmL in tubes containing de Man, Rogosa and Sharpe (MRS) medium. In particular, Weissella cibaria (P36) reached 10 CFUmL in MRS and was able to reduce the pH of the medium to 3.85. In the in vivo intestinal colonization studies, 72 healthy hybrid surubims were fed with a commercial diet supplemented with probiotic W. cibaria for 15 days. Changes in gut community composition were then analyzed, and probiotic pro&#64257;le of W. cibaria was determined molecularly by ampli&#64257;cation of rRNA 16S gene was performed using PCR. Compared to control &#64257;sh, W. cibaria-supplemented &#64257;sh showed an increase in RBC. These results show the ef&#64257;cacy of our haematological approach to probiotic screening in hybrid sorubim.
Palavras-Chave: | Fish nutrition; Lactic acid bacteria; sorubim; Weisella cibaria.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_37603.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
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