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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
27/10/2023 |
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Data da última atualização: |
27/10/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MANFIO, C. E.; MARO, L. A. C.; PASSOS, J. F. M.; COSTA, M. D. |
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Título: |
DIAGNÓSTICO DE DOENÇAS UTILIZANDO QPCR EM MUDAS CÍTRICAS REGENERADAS APÓS LIMPEZA CLONAL. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8., 2023, Florianópolis, SC. Resumos... São Paulo, SP: Sociedade Brasileira de Genética, 2023. p. 52 |
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Idioma: |
Inglês |
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Conteúdo: |
The citrus seedlings sector in Santa Catarina has experienced considerable expansion in recent years with an
increase in the number of seedlings produced and also in the number of families involved in the activity. The
lack of important diseases that commonly occur in other states of the federation could put the orchards in Santa
Catarina, the seedling producing region and also the experimental collections kept in the open by Epagri at
risk. In order for the activity to be profitable, whether as a fruit producer or seedling producer, it is essential
that the propagation materials used for the formation of seedlings are free of any pathogens. Therefore, the
diagnosis of diseases is extremely important, and clonal cleaning by micrografting is a primordial tool to ensure
the absence of viruses that are highly harmful to the exploitation of this crop. In view of the above, the objective
of this work was to certify the health of citrus seedlings regenerated after clonal cleaning. To obtain the
seedlings, buds from parent plants of the SCS454 Catarina, SCS455 Reinaldo, SCS 456 Sigmar, SCS 457
Souza and SCS458 Osvino cultivars were used. The qPCR technique was used to verify the micrografting
efficiency. Twenty-one seedlings were regenerated in vitro and had their RNA extracted using the Trizol
protocol. The qPCR reactions for analysis of CEVd (Citrus exocortis viroid) and HSV (Hop stunt viroid)
contained 1 μL of cDNA, 10 μL of master Mix (Sybr Green), 0.35 μM of primers, and water to make up the
final volume of 20 μL, and for the qPCR reactions for psorosis, the protocol by Francesco et al., 2014 was
used. Of the twenty-one seedlings, seventeen showed neither the presence of viroids nor Psorosis. The four
seedlings that were positive did not show both diseases together. Although the four seedlings were not
completely cleaned, they showed only one disease. Important results, since they confirm the efficiency of the
clonal cleaning technique through micrografting in obtaining basic material with phytosanitary quality. The
phytosanitary quality of the citrus seedling produced is a basic requirement for the frank development of the
activity and to place the seedling in Santa Catarina on a differentiated level, precisely because it does not occur
important diseases that are already diagnosed in other states of the federation. In this way, it will be possible
to transfer the benefits of biotechnology to the productive sector for the use of healthy propagation material by
nurserymen in the formation of seedlings and, consequently, contributing to the formation of long-lived
orchards. In this sense, the clonal cleaning technique by means of micrografting was efficient for obtaining
material free of viroids and psorisis. MenosThe citrus seedlings sector in Santa Catarina has experienced considerable expansion in recent years with an
increase in the number of seedlings produced and also in the number of families involved in the activity. The
lack of important diseases that commonly occur in other states of the federation could put the orchards in Santa
Catarina, the seedling producing region and also the experimental collections kept in the open by Epagri at
risk. In order for the activity to be profitable, whether as a fruit producer or seedling producer, it is essential
that the propagation materials used for the formation of seedlings are free of any pathogens. Therefore, the
diagnosis of diseases is extremely important, and clonal cleaning by micrografting is a primordial tool to ensure
the absence of viruses that are highly harmful to the exploitation of this crop. In view of the above, the objective
of this work was to certify the health of citrus seedlings regenerated after clonal cleaning. To obtain the
seedlings, buds from parent plants of the SCS454 Catarina, SCS455 Reinaldo, SCS 456 Sigmar, SCS 457
Souza and SCS458 Osvino cultivars were used. The qPCR technique was used to verify the micrografting
efficiency. Twenty-one seedlings were regenerated in vitro and had their RNA extracted using the Trizol
protocol. The qPCR reactions for analysis of CEVd (Citrus exocortis viroid) and HSV (Hop stunt viroid)
contained 1 μL of cDNA, 10 μL of master Mix (Sybr Green), 0.35 μM of pri... Mostrar Tudo |
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Thesagro: |
Key-words: Citrus spp; micrografting; real-time; seedling production. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03501naa a2200205 a 4500
001 1133863
005 2023-10-27
008 2023 bl uuuu u00u1 u #d
100 1 $aMANFIO, C. E.
245 $aDIAGNÓSTICO DE DOENÇAS UTILIZANDO QPCR EM MUDAS CÍTRICAS REGENERADAS APÓS LIMPEZA CLONAL.$h[electronic resource]
260 $c2023
520 $aThe citrus seedlings sector in Santa Catarina has experienced considerable expansion in recent years with an increase in the number of seedlings produced and also in the number of families involved in the activity. The lack of important diseases that commonly occur in other states of the federation could put the orchards in Santa Catarina, the seedling producing region and also the experimental collections kept in the open by Epagri at risk. In order for the activity to be profitable, whether as a fruit producer or seedling producer, it is essential that the propagation materials used for the formation of seedlings are free of any pathogens. Therefore, the diagnosis of diseases is extremely important, and clonal cleaning by micrografting is a primordial tool to ensure the absence of viruses that are highly harmful to the exploitation of this crop. In view of the above, the objective of this work was to certify the health of citrus seedlings regenerated after clonal cleaning. To obtain the seedlings, buds from parent plants of the SCS454 Catarina, SCS455 Reinaldo, SCS 456 Sigmar, SCS 457 Souza and SCS458 Osvino cultivars were used. The qPCR technique was used to verify the micrografting efficiency. Twenty-one seedlings were regenerated in vitro and had their RNA extracted using the Trizol protocol. The qPCR reactions for analysis of CEVd (Citrus exocortis viroid) and HSV (Hop stunt viroid) contained 1 μL of cDNA, 10 μL of master Mix (Sybr Green), 0.35 μM of primers, and water to make up the final volume of 20 μL, and for the qPCR reactions for psorosis, the protocol by Francesco et al., 2014 was used. Of the twenty-one seedlings, seventeen showed neither the presence of viroids nor Psorosis. The four seedlings that were positive did not show both diseases together. Although the four seedlings were not completely cleaned, they showed only one disease. Important results, since they confirm the efficiency of the clonal cleaning technique through micrografting in obtaining basic material with phytosanitary quality. The phytosanitary quality of the citrus seedling produced is a basic requirement for the frank development of the activity and to place the seedling in Santa Catarina on a differentiated level, precisely because it does not occur important diseases that are already diagnosed in other states of the federation. In this way, it will be possible to transfer the benefits of biotechnology to the productive sector for the use of healthy propagation material by nurserymen in the formation of seedlings and, consequently, contributing to the formation of long-lived orchards. In this sense, the clonal cleaning technique by means of micrografting was efficient for obtaining material free of viroids and psorisis.
650 $aKey-words: Citrus spp
650 $amicrografting
650 $areal-time
650 $aseedling production
700 1 $aMARO, L. A. C.
700 1 $aPASSOS, J. F. M.
700 1 $aCOSTA, M. D.
773 $tIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8., 2023, Florianópolis, SC. Resumos... São Paulo, SP: Sociedade Brasileira de Genética, 2023. p. 52
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Registro original: |
Epagri-Sede (Epagri-Sede) |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
30/05/2011 |
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Data da última atualização: |
30/05/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Circulação/Nível: |
-- - -- |
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Autoria: |
MOREIRA, I. C. L.; CERETTA, C. A.; GIROTTO, E.; TRENTIN, E. E.; POCOJESKI, E.; PANDOLFO, C. M. |
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Afiliação: |
Epagri |
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Título: |
Avaliações de perdas de nitrogênio e fósforo por lixiviação sob aplicação de dejetos de suínos em sucessão de culturas, durante três anos. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 15., 2004, Santa Maria, RS. Anais... Santa Maria, RS: SBCS; UFSM, 2004. |
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Idioma: |
Português |
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Conteúdo: |
Esse trabalho teve por objetivo avaliar as perdas de nitrogênio (N) e fósforo disponível na solução do solo, com aplicação de dejeto líquido de suínos. O experimento foi desenvolvido na Universidade Federal de Santa Maria no período de 05/2000 a 05/2003. O solo da área experimental é classificado como Argissolo Vermelho Arênico distrófico. Foram aplicadas quatro doses de dejeto líquido de suínos: 0, 20 40 e 80 m3 ha-1 antes da semeadura de cada espécie nas sucessões de aveia preta/milho/nabo forrageiro, durante os anos agrícolas 2000/01 e 2001/02 e na sucessão aveia preta/milheto/feijão durante o ano agrícola 2002/03. Os resultados sugerem não haver maiores problemas em relação a contaminação das águas de subsuperfície por N-NO3- e P com a utilização de até 40 m3 ha-1 de dejeto líquido de suínos. O somatório das perdas durante os três anos de cultivo foram insignificantes em relação a quantidade aplicada. |
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Palavras-Chave: |
Dejeto; Fósforo; Nitrato. |
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Categoria do assunto: |
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Marc: |
LEADER 01535naa a2200157 a 4500
001 1076962
005 2011-05-30
008 2004 bl uuuu u00u1 u #d
100 1 $aEpagri
245 $aAvaliações de perdas de nitrogênio e fósforo por lixiviação sob aplicação de dejetos de suínos em sucessão de culturas, durante três anos.
260 $c2004
520 $aEsse trabalho teve por objetivo avaliar as perdas de nitrogênio (N) e fósforo disponível na solução do solo, com aplicação de dejeto líquido de suínos. O experimento foi desenvolvido na Universidade Federal de Santa Maria no período de 05/2000 a 05/2003. O solo da área experimental é classificado como Argissolo Vermelho Arênico distrófico. Foram aplicadas quatro doses de dejeto líquido de suínos: 0, 20 40 e 80 m3 ha-1 antes da semeadura de cada espécie nas sucessões de aveia preta/milho/nabo forrageiro, durante os anos agrícolas 2000/01 e 2001/02 e na sucessão aveia preta/milheto/feijão durante o ano agrícola 2002/03. Os resultados sugerem não haver maiores problemas em relação a contaminação das águas de subsuperfície por N-NO3- e P com a utilização de até 40 m3 ha-1 de dejeto líquido de suínos. O somatório das perdas durante os três anos de cultivo foram insignificantes em relação a quantidade aplicada.
653 $aDejeto
653 $aFósforo
653 $aNitrato
773 $tIn: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 15., 2004, Santa Maria, RS. Anais... Santa Maria, RS: SBCS; UFSM, 2004.
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