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| Registros recuperados : 9 | |
| 1. |  | ZAGO, F. C.; GAUDÊNCIO NETO, S.; MARINHO, L. R. S.; SCHUTZ, L. F.; AGUIAR, L. H.; ZARDO, V. F.; MEZZALIRA, A.; BERTOLINI, M. Aspiração folicular transvaginal como ferramenta para obtenção de gametas em vacas da raça flamenga (dados preliminares). In: SEMINÁRIO REGIONAL DOS PÓS-GRADUANDOS EM CIÊNCIAS AGRÁRIAS, 3., 2010, Lages, SC. Anais... Lages, SC: UDESC, 2010.| Biblioteca(s): Epagri-Sede. |
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| 2. | | MEZZALIRA, J. C.; OHLWEILER, L. U.; URIO, M.; GAUDENCIO NETO, S.; MARINHO, L. R. S.; ZAGO, F. C.; FORELL, F.; BERTOLINI, M.; MEZZALIRA, A. Effect of nitrocooler negative pressure and recovery internal on cryotolerance of bovine in vitroproduced embryos. In: ANNUAL CONFERECE OF IETS, ., 2010, Cordoba, Argentina. Proceedings... Sydney, Australia: CSIRO, 2010. p. 210.| Biblioteca(s): Epagri-Sede. |
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| 3. | | ZAGO, F. C.; GAUDENCIO NETO, S.; MARINHO, L. R. S.; SCHUTZ, L. F.; AGUIAR, L. H.; ZARDO, V. F.; MEZZALIRA, A.; BERTOLINI, M. Efficiency of reproductive technologies applied to the flamenga breed for genetic conservation (PRELIMINARY DATA). In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIOES, 24., 2010, Porto de Galinhas, PE. Anais... Porto Alegre, RS: UFRGS, 2010. p. 770.| Biblioteca(s): Epagri-Sede. |
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| 4. | | CASALI, R.; ZAGO, F. C.; FEDERLE, M.; DRUM, J. N.; SPONCHIADO, M.; KLEIN, N.; ZANETTI, M. S.; GAUDÊNCIO NETO, S.; MEZZALIRA, A.; COSTA, U. M.; VIEIRA, A. D. Lyophilized seminal plasma improves the motility of frozen RAM semen and increases the cleavage in heterologous IVF. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco, CE . [Proceedings...]. Porto Alegre, RS: UFRGS, 2011. p. 356.| Biblioteca(s): Epagri-Sede. |
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| 5. | | ZAGO, F. C.; GAUDENCIO NETO, S.; AGUIAR, L. H.; SCHUTZ, L. F.; FORELL, F.; MARINHO, L. R. S.; MACHADO JÚNIOR, J.; MEZZALIRA, A.; BERTOLINI, M. Ovum pickup-in vitro production efficiency for conservation of the flamenga cattle breed. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2011, Orlando, FL, EUA. Proceedings... Sydney, Australia: CSIRO, 2011. p. 213-214.| Biblioteca(s): Epagri-Sede. |
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| 6. | | MARTINS, L.T.; SANTOS NETO, P.C. dos; GAUDÊNCIO NETO, S.; RAUBER, L.P.; BERTOLINI, M.; VIEIRA, A.D.; MEZZALIRA, A. Microbiological and functional evaluation of an alternative device (OB)for estrous synchonization in ewes. Ciência Rural, Santa Maria, RS, V. 40, n. 2, p. 389-395, fev. 2010.| Biblioteca(s): Epagri-Sede. |
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| 7. | | GAUDÊNCIO NETO, S.; MARTINS, L. T.; ORTIGARI JÚNIOR, I.; ZAGO, F. C.; CASALI, R.; SANTOS NETO, P. C.; FELTRIN, C.; MAIA FILHO, S. P.; CASTRO, E. V.; NUNES, J. F.; BERTOLINI, M.; MEZZALIRA, A. Adição de plasma seminal equino liofilizado no reduz a taxa de degradação da motilidade do sêmen ovino congelado. In: REUNIÃO ANUAL DA SOCIEDADE BARSILEIRA DE TECNOLOGIA DE EMBRIÔES, 24., 2010, Porto de Galinhas, PE. Anais... Porto Alegre: UFRGS, 2010. p. 782-782.| Biblioteca(s): Epagri-Sede. |
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| 8. | | SCHTZ, L. F.; ZAGO, F. C.; AGUIAR, L. H.; SANTOS NETO, P. C.; MACHADO JÚNIOR, J.; URIO, M.; MARTINS, L. T.; FERNANDES, D.; BRAUN, W.; GAUDÊNCIO NETO, S.; FELTRIN, C.; BERTOLINI, L. R.; COSTA, U. M.; FORELL, F.; BERTOLINI, M. Changes in blood chemistry profiles and association with birthweights in IVF-derived newborn calves. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco, CE. Acta Scientiea Veterinariae. Porto Alegre, RS: UFRGS, 2011. p. 418.| Biblioteca(s): Epagri-Sede. |
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| 9. | | ORTIGARI JÚNIOR, I.; GERGER, R. P. C.; RIBEIRO, E. S.; MEZZALIRA, J. C.; ZAGO, F. C.; GAUDÊNCIO NETO, S.; OHLWEILER, L. U.; MARTINS, L. T.; BERTOLINI, L. R.; FORELL, F.; COSTA, U. M.; LOPES, R. F. F.; RODRIGUES, J. L.; AMBRÓSIO, C. E.; MIGLINO, M. A.; MEZZALIRA, A.; BERTOLINI, M. Efeito da evolução técnica na eficiência na clonagem por handmade cloning em bovinos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILERIA DE TECNOLOGIA DE EMBRIÕES, 24., 2010, Porto de Galinhas, PE. Anais... Porto Alegre, RS: UFRGS, 2010. p. 803.| Biblioteca(s): Epagri-Sede. |
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| Registros recuperados : 9 | |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
10/10/2011 |
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Data da última atualização: |
10/10/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Circulação/Nível: |
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Autoria: |
CASALI, R.; ZAGO, F. C.; FEDERLE, M.; DRUM, J. N.; SPONCHIADO, M.; KLEIN, N.; ZANETTI, M. S.; GAUDÊNCIO NETO, S.; MEZZALIRA, A.; COSTA, U. M.; VIEIRA, A. D. |
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Afiliação: |
Epagri |
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Título: |
Lyophilized seminal plasma improves the motility of frozen RAM semen and increases the cleavage in heterologous IVF. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco, CE . [Proceedings...]. Porto Alegre, RS: UFRGS, 2011. p. 356. |
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Idioma: |
Português |
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Conteúdo: |
The addition of heterologous seminal plasma (HSP) to the freezing extender of ram semen improved post-thaw viability parameters, and might be an alternative to improve intra-cervical insemination and in vitro fertilization (IVF) with frozen ram semen. However, data regarding such conditions are still very scarce, especially for lyophilized plasma. The aim of this study was to evaluate the effect of addition of lyophilized bovine seminal plasma (LBSP) and equine (LESP) to the freezing extender of ram semen. The evaluations performed were: progressive motility (PM) after thaw (AT) and PM after percoll selection (PS), acrosome integrity (AI) after PS and cleavage rates after IVF with heterologous bovine oocytes. Seminal plasma was obtained from five bulls and five stallions collected with artificial vagina. The ejaculates were centrifuged (5000 rpm) and lyophilized with Christ Alpha 1-4 Freeze Dryer. The ram semen was collected with artificial vagina from four Texel rams, pooled and diluted in 1 +1 in glycerolated Tris-egg yolk without seminal plasma (Tris control, TC) or added of 600¥ìg/mL of LBSP or 600¥ìg/mL of LESP. The semen was then cooled at 0.3 ¡ÆC / min. until to 5 ¨¬C, stabilized for one hour, loaded in 0.25 mL straws in isothermal conditions and frozen at -80 ¡ÆC in LN2 vapor before store in cryogenic container at -196 ¡ÆC. The sperm viability was determined based on the PM-AT and PM-PS. The sperm were used for heterologous IVF (Garcia-Alvarez, 2009, Theriogenology, 71: 643-650), and to evaluate the acrosome reaction by FITC method (Sukardi Ani Sci Rep 1997, 46: 89-96). Data were analyzed by Student T test with significance level of 5%. There was no difference between the average rates of post thaw progressive motility PM-AT of PSLB (27.5%) and PSLE (37.5%) groups, but both were higher than TC group (10%), improving the viability of thawed semen by the addition of heterologous seminal plasma. The rates of PM-PS were similar in all groups (TC 40.0%, 56.7% LBSP, LESP 70.0%). There was no difference in the IAPP evaluation of different groups (CT 58.4%, 67.3% LBSP, LESP 63.3%). There was a significant increase in cleavage rate of LBSP (45.7%) and LESP (43.7%) groups when compared to TC group (32.6%). The increased cleavage rate observed in heterologous IVF demonstrates that the frozen semen added of LEAP or LBSP has a greater oocyte penetration ability, which suggests that they may be more efficient for sheep artificial insemination. MenosThe addition of heterologous seminal plasma (HSP) to the freezing extender of ram semen improved post-thaw viability parameters, and might be an alternative to improve intra-cervical insemination and in vitro fertilization (IVF) with frozen ram semen. However, data regarding such conditions are still very scarce, especially for lyophilized plasma. The aim of this study was to evaluate the effect of addition of lyophilized bovine seminal plasma (LBSP) and equine (LESP) to the freezing extender of ram semen. The evaluations performed were: progressive motility (PM) after thaw (AT) and PM after percoll selection (PS), acrosome integrity (AI) after PS and cleavage rates after IVF with heterologous bovine oocytes. Seminal plasma was obtained from five bulls and five stallions collected with artificial vagina. The ejaculates were centrifuged (5000 rpm) and lyophilized with Christ Alpha 1-4 Freeze Dryer. The ram semen was collected with artificial vagina from four Texel rams, pooled and diluted in 1 +1 in glycerolated Tris-egg yolk without seminal plasma (Tris control, TC) or added of 600¥ìg/mL of LBSP or 600¥ìg/mL of LESP. The semen was then cooled at 0.3 ¡ÆC / min. until to 5 ¨¬C, stabilized for one hour, loaded in 0.25 mL straws in isothermal conditions and frozen at -80 ¡ÆC in LN2 vapor before store in cryogenic container at -196 ¡ÆC. The sperm viability was determined based on the PM-AT and PM-PS. The sperm were used for heterologous IVF (Garcia-Alvarez, 2009, Theriogenology, ... Mostrar Tudo |
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Palavras-Chave: |
Cryopreservation; Heterologous seminal plasma; Lyophilization. |
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Categoria do assunto: |
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Marc: |
LEADER 03086naa a2200157 a 4500
001 1081168
005 2011-10-10
008 2011 bl uuuu u00u1 u #d
100 1 $aEpagri
245 $aLyophilized seminal plasma improves the motility of frozen RAM semen and increases the cleavage in heterologous IVF.
260 $c2011
520 $aThe addition of heterologous seminal plasma (HSP) to the freezing extender of ram semen improved post-thaw viability parameters, and might be an alternative to improve intra-cervical insemination and in vitro fertilization (IVF) with frozen ram semen. However, data regarding such conditions are still very scarce, especially for lyophilized plasma. The aim of this study was to evaluate the effect of addition of lyophilized bovine seminal plasma (LBSP) and equine (LESP) to the freezing extender of ram semen. The evaluations performed were: progressive motility (PM) after thaw (AT) and PM after percoll selection (PS), acrosome integrity (AI) after PS and cleavage rates after IVF with heterologous bovine oocytes. Seminal plasma was obtained from five bulls and five stallions collected with artificial vagina. The ejaculates were centrifuged (5000 rpm) and lyophilized with Christ Alpha 1-4 Freeze Dryer. The ram semen was collected with artificial vagina from four Texel rams, pooled and diluted in 1 +1 in glycerolated Tris-egg yolk without seminal plasma (Tris control, TC) or added of 600¥ìg/mL of LBSP or 600¥ìg/mL of LESP. The semen was then cooled at 0.3 ¡ÆC / min. until to 5 ¨¬C, stabilized for one hour, loaded in 0.25 mL straws in isothermal conditions and frozen at -80 ¡ÆC in LN2 vapor before store in cryogenic container at -196 ¡ÆC. The sperm viability was determined based on the PM-AT and PM-PS. The sperm were used for heterologous IVF (Garcia-Alvarez, 2009, Theriogenology, 71: 643-650), and to evaluate the acrosome reaction by FITC method (Sukardi Ani Sci Rep 1997, 46: 89-96). Data were analyzed by Student T test with significance level of 5%. There was no difference between the average rates of post thaw progressive motility PM-AT of PSLB (27.5%) and PSLE (37.5%) groups, but both were higher than TC group (10%), improving the viability of thawed semen by the addition of heterologous seminal plasma. The rates of PM-PS were similar in all groups (TC 40.0%, 56.7% LBSP, LESP 70.0%). There was no difference in the IAPP evaluation of different groups (CT 58.4%, 67.3% LBSP, LESP 63.3%). There was a significant increase in cleavage rate of LBSP (45.7%) and LESP (43.7%) groups when compared to TC group (32.6%). The increased cleavage rate observed in heterologous IVF demonstrates that the frozen semen added of LEAP or LBSP has a greater oocyte penetration ability, which suggests that they may be more efficient for sheep artificial insemination.
653 $aCryopreservation
653 $aHeterologous seminal plasma
653 $aLyophilization
773 $tIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco, CE . [Proceedings...]. Porto Alegre, RS: UFRGS, 2011. p. 356.
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