Data/hora: 01/06/2024 08:00:57

Biblioteca(s): 	Epagri-Sede.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	-- - --	
Data corrente: 	28/04/2011	
Data da última atualização: 	28/04/2011	
Autoria: 	LEITE, G. B.; FINARDI, N. L.; FORTES, G. R. L.	
Afiliação: 	Epagri	
Título: 	Use of vermiculite as a substrate and effect of light on in vitro rooting of pears, cv. Bartlett and clone OHxF 97.	
Ano de publicação: 	2002	
Fonte/Imprenta: 	Ciência e Agrotecnologia, Lavras, v. 26, n. 5, p. 977-982, 2002.	
Idioma: 	Português	
Notas: 	ISSN, 1413-7054	
Conteúdo: 	 Agar is traditionally used to solidify medium in cultures grown on in vitro conditions.
However, it has been observed, in some cases, a poor root development, along with the non-functionality of these roots when transferred to in vivo conditions. Two trials were carried out at Embrapa Clima Temperado laboratory with pears (Pyrus sp) cv. Bartlett and OH x F97 clone), to investigate the possibility of replacing agar by vermiculite as a physical support, and to analyze the influence of light on the base of micro cuttings on
root development. Shoots were conditioned in a medium MS/3 to which was added myoinositol (100 mg.L-1; sucrose (30 g.L-1); agar (6 g.L-1) or vermiculite (8g/tube).
No growth regulator was added. The darkening treatment was accomplished by putting a black sheet on the top of the agar medium. The evaluation was performed after 30 days on the shoots being maintained on 25 °C, 2000 lux and 16 hour of photoperiod. The trial
was performed in a completely ramdomized block and the means were compared by using the Duncan test at 5% probability. Either agar or vermiculite did not affect rooting percentage. On the other hand, shoots on agar showed a higher number of roots per shoot for both ´ Bartlett` and OH x F97 clone. Root hairs were not observed on agar grown plantlets, but they were produced on vermiculite grown ones. As secondary root
is concerned, it was not observed any on agar grown shoots, whereas shoots rooted in vermiculite had around 31% and 53% of secondary root for ´Bartlett and OH x F97 clone, respectively. Darkened medium decreased the percentage of rooted plantlets from 98 to
86 and the root length from 3.5 to 2.1 cm.
	
Palavras-Chave: 	Agar; Micropropagation; Pyrus sp; Root quality; Tissue culture.	
Categoria do assunto: 	--	
URL: 	http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_1208.pdf	
Registro original: 	Epagri-Sede (Epagri-Sede)	
Exemplares: 	Epagri-Sede - (78679-1) - UPC - AP - -- -  -  -  - DIS