Data/hora: 18/06/2024 08:36:49

Biblioteca(s): | Epagri-Sede.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | Internacional - B
Data corrente: | 17/01/2020
Data da última atualização: | 17/01/2020
Autoria: | YAMASHITA, M. M.; FERRAREZI, J. V.; PEREIRA, G. V.; BANDEIRA JÚNIOR, G.; SILVA, B. C.; PEREIRA, S. A.; MARTINS, M. L.; MOURIÑO, J. L. P.
Título: | Autochthonous vs allochthonous probiotic strains to Rhamdia quelen.
Ano de publicação: | 2020
Fonte/Imprenta: | Microbial Pathogenesis, Amsterdã, Países Baixos, n. 139, p. 103897, 2020.
Idioma: | Inglês
Conteúdo: | The aim of this study was to obtain an autochthonous probiotic candidate strain from the silver cat&#64257;sh (Rhamdia quelen) intestinal tract, comparing its in vivo performance with an allochthonous probiotic isolated from another &#64257;sh, Nile tilapia (Oreochromis niloticus), in a growth performance assay. The study was divided in two parts: in vitro and in vivo assay followed by challenge with A. hydrophila. In the in vitro assay, the species-speci&#64257;c isolated strain Lactococcus lactis presented characteristics such as: absence of hemolysis, antagonism to bacterial pathogens isolated from freshwater &#64257;sh, and considerable speed of duplication. In the in vivo trial, both &#64257;sh supplemented with autochthonous or allochthonous strains presented an increase the &#64257;nal concentration of lactic acid bacteria in the intestinal tract of the &#64257;sh after 60 days of dietary supplementation reaching con- &#8722;1 &#8722;1 7 7 centrations of 1 × 10 CFU g and 4 × 10 UFC.g , respectively. In addition, the autochthonous strain increased the mean corpuscular hemoglobin (MCH) of the treated animals, but no signi&#64257;cant di&#64256;erences were observed in the other hemato-immunological and zootechnical parameters between treatments. After challenge with Aeromonas hydrophila, only animals that received autochthonous probiotic supplementation showed an increase in the serum total immunoglobulin concentration, but not enough to observe a signi&#64257;cant di&#64256;erence in the survival rate between the treatments. Dietary supplementation of the probiotic allochthonous strain did not demonstrate any e&#64256;ects superior to those of the isolated autochthonous strain. Although the autochthonous strain did not present signi&#64257;cant improvements in the other parameters evaluated in this study, it was able to inhibit bacterial pathogens in vitro, to increase the &#64257;nal concentration of LAB's and the amount of im- munoglobulin after experimental challenge, demonstrating probiotic potential. This study demonstrated for the &#64257;rst time the isolation and in vivo use of an autochthonous probiotic strain isolated from silver cat&#64257;sh, as well as its comparative evaluation with the performance of allochthonous probiotic.
Palavras-Chave: | Disease resistance; Immunoglobulin; Lactic acid bacteria; Lactobacillus plantarum; Lactococcus lactis; sh; Silver cat&#64257.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_44550.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (104389-1) - UPC - AP - DD -  -  -  - DIS