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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
17/08/2021 |
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Data da última atualização: |
17/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAÚJO, E. R.; RESENDE, R. S.; LIMA, M. F. |
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Título: |
First report of Iris yellow spot orthotospovirus infecting onion in Santa Catarina State, Brazil. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Summa Phytopathologica, Botucatu, v. 47, n. 2, p. 131-133, 2021. |
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Idioma: |
Português |
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Conteúdo: |
Onion (Allium cepa L.) is the third most economically important vegetable crop in Brazil; its planted area was estimated at approximately 48,000 hectares with total production of 1,556,885 tons in 2019. Santa Catarina is the largest national producing state, accounting for 532,000 tons in the 2019/20 crop season. Iris yellow spot orthotospovirus (IYSV), genus Orthotospovirus, family Tospoviridae, is transmitted by Thrips tabaci L. in a persistent and propagative manner. Frankliniella fusca can also transmit IYSV but less efficiently. IYSV has been reported in South, Central and North America, Africa, Europe, Asia, and Oceania. In Brazil, the first report of IYSV-like symptoms in onion fields occurred in 1981, in Rio Grande do Sul State, and the causal agent was initially identified as tomato spotted wilt-like virus. However, correct identification of the pathogen as IYSV, causing the disease known as ?sapeca?, was obtained only in 1994, when disease symptoms were observed in onion fields in the sub-middle region of São Francisco River Valley ? Pernambuco and Bahia States. Since the 1990s, no new reports on the occurrence of IYSV infecting onion fields in the country have been made. During 2017/18, 2018/19 and 2019/20 crop seasons, sudden leaf blight symptoms were observed in bulb onion crops in open fields in Santa Catarina State. Eventually, leaves exhibited whitish diamond-shaped spots, characteristic of IYSV-like symptoms. In turn, yellow or light green diamond-shaped spots, which may or may not have a lighter center, were observed on scape from seed onion crop. Sample collection was performed considering three crop seasons: 2017/18: 63 leaf samples were collected ? 56 from symptomatic onions and seven from weeds showing leaf spots [Bidens pilosa (three samples), Amaranthus sp. (two samples), Raphanus sp. (one sample), and Euphorbia heterophylla (one sample)]; 2018/19: 12 leaf samples were collected ? seven from symptomatic and five from asymptomatic onion plants; 2019/20: 30 leaf samples were collected ? all from symptomatic onion plants. Samples were evaluated by NCMELISA (Nitrocellulose Membrane-enzyme-linked immunosorbent assay) with polyclonal antibodies raised against the nucleoprotein of IYSV (produced at the National Center for Vegetable Crops Research ? CNPH) or using IYSV ImmunoStrips (Agdia Inc., Elkhart, IN) or even by DAS-ELISA (Double Antibody Sandwich enzyme-linked immunosorbent assay), according to the manufacturer?s instructions (Agdia Inc., Elkhart, IN). For each serological test, the sample was considered positive, or virus-infected, compared to the reaction of the positive control (NCM-ELISA: formation of precipitate by the action of enzymatic reaction on the BCIP and NBT, resulting in purplish spots; ImmunoStrips: appearance of a dark line in the positive control; DAS-ELISA: absorbance reading of the sample was at least twice the mean absorbance value recorded for the extract of a healthy plant used as negative control). Out of 105 field samples analyzed, 33 (31.4%) had a positive signal to IYSV antibodies, compared to the negative and IYSV-positive controls. It is worth pointing out that all IYSV-positive samples were collected from symptomatic onion plants. Besides, no leaf samples obtained from symptomless onion plants or weed species tested positive. IYSV has been detected in six counties in Santa Catarina State. Interestingly, samples collected in all three crop seasons led to similar infection rates. Among IYSV-positive samples, 20 were collected in the 2017/18 crop season (11 from Ituporanga, five from Leoberto Leal, one from Alfredo Wagner, one from Aurora, one from Atalanta, and one from Canoinhas) and showed an infection rate of 31.74%. Leaf samples collected in the 2018/19 crop season also tested positive for IYSV (two from Aurora and two from Ituporanga), resulting in an infection rate of 33.33%. Nine samples from the last crop season (2019/2020) also reacted positively with IYSV antibodies (all from Ituporanga County), producing an infection rate of 30.0%. Considering that the collection of field samples focused on symptomatic plants, the overall detection rate (31.4%) can be regarded as relatively low; however, possible justification for symptomatic samples that tested negative to specific IYSV polyclonal antibodies (68.6%) are: i) The plants were not infected with IYSV and the observed symptoms were due to other causes, such as abiotic factors, since not all of them had diamond-shaped lesions, a characteristic symptom of this disease. ii) Sampling: differently from other orthotospovirus species, IYSV moves less systemically in the infected plant and tends to cause localized infection. The virus does not present an even distribution in the infected plant, and high virus titers are usually found for inner leaves. In addition, the virus may not be found in all leaves. IYSV tends to be localized in and in close proximity to the lesions on leaves and scapes. It is frequently detected in visible lesions of at least 30-50 mm. Thus, the portion of the leaf sample used for serological analysis could not contain enough viral particles for detection by ELISA, which may eventually result in false negatives. iii) The plants could be infected with other viruses and not with IYSV. All positive and negative controls used in the serological tests (NCM-ELISA; DAS-ELISA) reacted as expected. IYSV may become an emerging virus to onion crops in Brazil as it is in other regions of the world. Monitoring onion fields is mandatory to learn about the geographical distribution, spread and occurrence of this disease. To our knowledge, this is the first report of Iris yellow spot orthotospovirus infecting onion in Santa Catarina State. MenosOnion (Allium cepa L.) is the third most economically important vegetable crop in Brazil; its planted area was estimated at approximately 48,000 hectares with total production of 1,556,885 tons in 2019. Santa Catarina is the largest national producing state, accounting for 532,000 tons in the 2019/20 crop season. Iris yellow spot orthotospovirus (IYSV), genus Orthotospovirus, family Tospoviridae, is transmitted by Thrips tabaci L. in a persistent and propagative manner. Frankliniella fusca can also transmit IYSV but less efficiently. IYSV has been reported in South, Central and North America, Africa, Europe, Asia, and Oceania. In Brazil, the first report of IYSV-like symptoms in onion fields occurred in 1981, in Rio Grande do Sul State, and the causal agent was initially identified as tomato spotted wilt-like virus. However, correct identification of the pathogen as IYSV, causing the disease known as ?sapeca?, was obtained only in 1994, when disease symptoms were observed in onion fields in the sub-middle region of São Francisco River Valley ? Pernambuco and Bahia States. Since the 1990s, no new reports on the occurrence of IYSV infecting onion fields in the country have been made. During 2017/18, 2018/19 and 2019/20 crop seasons, sudden leaf blight symptoms were observed in bulb onion crops in open fields in Santa Catarina State. Eventually, leaves exhibited whitish diamond-shaped spots, characteristic of I... Mostrar Tudo |
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Palavras-Chave: |
Allium cepa; ocorrência; virose. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
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Registro original: |
Epagri-Sede (Epagri-Sede) |
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| Registros recuperados : 458 | |
| 2. |  | MENEZES JÚNIOR, F. O. G.; VIEIRA NETO, J.; KURTZ, C. Crescimento, desenvolvimento, produtividade e perda pós-colheita da cebola em função de podas na fase de produção de mudas. Revista de Ciências Agroveterinárias, Lages, SC, v. 12, n. 2, p. 141-148, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Epagri-Sede. |
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| 5. | | SILVA, E.; GANDIN, C. L.; DEBARBA, J. F. Onion Production for export for export in Santa Catarina State, South Brazil. Onion Newsletter For the Tropics, Chatham Maritme, Kent, v. 1, n. 4, p. 16-18, 1992.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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| 10. | | ALVES, D. P.; MANFIO, C. E.; ARAÚJO, E. R.; HIGASHIKAWA, F. S. ANÁLISE DO CITOPLASMA E DO GENE RESTAURADOR DA MACHOFERTILIDADE NO CULTIVAR DE CEBOLA MEGA®. In: SEMINÁRIO CATARINENSE DE OLERICULTURA, 2., 2024, Fraiburgo, SC. Resumos... Caçador, SC: Epagri, 2024. p. 295| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 11. | | ALVES, D. P.; MANFIO, C. E.; ARAÚJO, E. R.; HIGASHIKAWA, F. S. ANÁLISE DO CITOPLASMA E DO GENE RESTAURADOR DA MACHOFERTILIDADE NO CULTIVAR DE CEBOLA PRINCESA DO SUL®. In: SEMINÁRIO CATARINENSE DE OLERICULTURA, 2., 2024, Fraiburgo, SC. Resumos... Caçador, SC: Epagri, 2024. p. 296| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 13. | | LANZENDORF, D. Z.; GIOVANETTI, L. K.; ZIMMERMANN, M. L. S.; VAES, L. M.; KURTZ, C.; COMIN, J. J.; LOVATO, P. E. GLOMALIN FRACTIONS IN ONION NO-TILL SYSTEM. In: CONGRESSO LATINO-AMERICANO DE CIÊNCIA DO SOLO, 23., CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 38., 2023, Florianópolis, SC. Resumos... Florianópolis, SC: Epagri, 2023. p. 256| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 16. | | OLIVEIRA, R. A.; BRUNETTO, G.; LOSS, A.; GATIBONI, L. C.; KURTZ, C.; MÜLLER JÚNIOR, V.; LOVATO, P. E.; OLIVEIRA, B. S.; SOUZA, M.; COMIN, J. J. Cover Crops Effects on Soil Chemical Properties and Onion Yield. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 40, n. , p. 1-17, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
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| 17. | | KURTZ, C.; PAULETTI, V.; ERNANI, P. R.; MENEZES JÚNIOR, F. O. G. Rendimento e conservação de cebola influenciados pela dose e parcelamento de nitrogênio no sistema de plantio direto. In: CONGRESSO BRASILEIRO DE SOLOS, 34., 2013, Florianópolis, SC. [Anais...]. Florianópolis, SC: SBCS, 2013.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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| Registros recuperados : 458 | |
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