Data/hora: 16/06/2024 08:48:52

Biblioteca(s): 	Epagri-Sede.	
Data corrente: 	25/01/2017	
Data da última atualização: 	25/01/2017	
Autoria: 	BRAMORSKI, A.; CRISTOFOLINI, A. F.; VITORINO, J. C.; MELO, S. S.; MATIAS, V. A.; ZAMBONIM, F. M.; VISCONTI, A.; SILVA JÚNIOR, A. A.	
Título: 	SINGLE CELL GEL ELECTROPHORESIS TO STUDY THE GENOTOXIC POTENCIAL OF Hibiscus cannabinus.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	In: SIMPÓSIO IBERO-AMERICANO DE PLANTAS MEDICINAIS , 8., 2016, Itajaí, SC. Anais. Itajaí: Univali, 2016. p. 517	
Idioma: 	Inglês	
Conteúdo: 	The specie Hibiscus cannabinus, popularly known as kenaf is a native plant from India and Africa, used in the popular medicine for the treatment of inflammations, diarrhea and blood disorders. Research has shown that this plant has an important variety of active components such as alkaloids, saponins, tannins and polyphenols with widely studied functions, but studies related to the potential toxicity of these compounds are still necessary. Objective: In this sense, the objective of this research was to determine the genotoxic activity from the extract of dried flowers of Hibiscus cannabinus in vitro grown at the Santa Catarina State Research Company - EPAGRI in the city of Itajai. Methods:The evaluation from the genotoxicity was performed using the comet assay with DNA of human leukocyte cells exposed to different concentrations of the sample from H. cannabinus (2 mg/mL, 1 mg/mL, 0.5 mg/mL, 0.25 mg/mL, 0.05 mg/mL) during periods of 1, 12 and 24 hours in order to evaluate the presence of cellular DNA damage). Results and Discussion: The results showed that none of the tested concentrations generated significant increase in DNA damage compared to the control group (PBS). In most cells analyzed was not observed the presence of tail nucleoids (Class 0). Few cells showed damage suffering minor damage (class 1) and few cells suffered more damage (class 2 and 3). Also, there was no significant difference between these concentrations and exposure times. Conclusion: In this way, the comet assay indicated that flower extract from H. cannabinus did not lead to DNA damage of human leukocytes in vitro. Considering the important role of diet on the modulation of DNA damage becomes relevant the research from the possible biological effects of plants with little studies in the scientific community.	
Palavras-Chave: 	genotoxicidade; Hibiscus cannabinus.	
Categoria do assunto: 	K Ciência Florestal e Produtos de Origem Vegetal	
URL: 	http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_38774.pdf	
Registro original: 	Epagri-Sede (Epagri-Sede)	
Exemplares: 	Epagri-Sede - (100942-1) - UPC - PL - DD -  -  -  - DIS