Data/hora: 26/06/2024 09:33:33

Biblioteca(s): | Epagri-Sede.
Data corrente: | 09/07/2015
Data da última atualização: | 09/07/2015
Autoria: | FERREIRA, R. C. U.; VIDIGAL FILHO, P. S.; VIDIGAL, M. C. G.; MOIANA, L. D.; KVITSCHAL, M. V.
Título: | Genetic and population structure of sweet cassava Manihot esculenta Crantz germplasm collected from Campo Grande Mato Grosso do Sul Brazil.
Ano de publicação: | 2015
Fonte/Imprenta: | Australian Journal of Crop Science, Austrália, v. 9, n. 5, p. 458-467, 2015.
Idioma: | Inglês
Conteúdo: | Knowledge of germplasm diversity and its genetic characterization is crucial to crop improvement and conservation strategies. This study aimed at characterization of diversity genetic and population structure of traditional sweet cassava germplasm at the molecular level. A total of 60 sweet cassava accessions from Campo Grande city, Mato Grosso do Sul State, Brazil, and a commercial variety, were analyzed using  microsatellite  markers. Allelic  frequencies  were used in order to assess the genetic diversity indexes for each marker. Population structure was analyzed using genetic distances and Bayesian model. A total of 88 alleles were identified from the 19  SSR  markers,  with  an  average  of  4.63  alleles  per marker.  The  mean  of  polymorphic  information  content  (PIC)  was  0.525 and indicated  that  the markers used  were  informative  and  polymorphic,  especially  the marker SSRY  13.  The  heterozygosity  observed (Ho)  ranged  from  0.569  to 0.984,  with  a  mean  of  0.862,  evidencing  the  existence  of  high  natural  heterozygosis  in  cassava.  The genetic diversity mean was of 0.601 and revealed considerable genetic diversity among the genotypes. Population structure analysis resulted  in  the  distribution  of  the  61  accessions  in  two  distinct sub-populations,  by  Bayesian analysis,  Neighbor-Joining  Tree  and Principal Coordinates  Analysis, indicating  the  existence  of  significant  genetic  diversity  between  sub-populations. Combination  of cassava accessions BGM 570 × BGM 613 and BGM 570 × IAC 576/70 is recommended for obtaining superior segregation in order to improve yield. The results will contribute to the conservation of the Mato Grosso do Sul germplasm and sweet cassava breeding.
Palavras-Chave: | genetic diversity; Manihot esculenta Crantz; microsatellite markers; populations structure; sweet cassava.
Categoria do assunto: | G Melhoramento Genético
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_36447.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (99153-1) - UPC - AP - DD -  -  -  - DIS