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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
06/05/2010 |
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Data da última atualização: |
06/05/2010 |
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Autoria: |
ROSA, P. M.; CAMPOS, T. de; SOUSA, E. C. B. de; SFORÇA, D. A.; TORRES, G. A. M.; SOUZA, A. P. de. |
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Título: |
Potato cultivar identification using molecular markers. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 1, p. 110-113, jan. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
O objetivo deste trabalho foi avaliar um conjunto de marcadores microssatélites para identificação e caracterização varietal das cultivares de batata mais amplamente utilizadas no Brasil. O DNA das 14 variedades de batata foi genotipado com marcadores microssatélites, e os alelos foram visualizados em gel de poliacrilamida corado com prata. Vinte e quatro marcadores foram avaliados e apenas um loco foi monomórfico. Com base no padrão de bandas, foi obtido um conjunto com dois microssatélites capazes de identificar e diferenciar todas as cultivares analisadas. |
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Palavras-Chave: |
Certificação genética; Identificação varietal; Marcador molecular; Proteção varietal; Solanum tuberosum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01285naa a2200241 a 4500
001 1069537
005 2010-05-06
008 2010 bl uuuu u00u1 u #d
100 1 $aROSA, P. M.
245 $aPotato cultivar identification using molecular markers.
260 $c2010
520 $aO objetivo deste trabalho foi avaliar um conjunto de marcadores microssatélites para identificação e caracterização varietal das cultivares de batata mais amplamente utilizadas no Brasil. O DNA das 14 variedades de batata foi genotipado com marcadores microssatélites, e os alelos foram visualizados em gel de poliacrilamida corado com prata. Vinte e quatro marcadores foram avaliados e apenas um loco foi monomórfico. Com base no padrão de bandas, foi obtido um conjunto com dois microssatélites capazes de identificar e diferenciar todas as cultivares analisadas.
653 $aCertificação genética
653 $aIdentificação varietal
653 $aMarcador molecular
653 $aProteção varietal
653 $aSolanum tuberosum
700 1 $aCAMPOS, T. de
700 1 $aSOUSA, E. C. B. de
700 1 $aSFORÇA, D. A.
700 1 $aTORRES, G. A. M.
700 1 $aSOUZA, A. P. de.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 45, n. 1, p. 110-113, jan. 2010.
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
20/07/2012 |
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Data da última atualização: |
20/07/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
-- - -- |
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Autoria: |
COGO, K.; ANDRADE, A.; LABATE, C. A.; BERGAMASCHI, C. C.; BERTO, L. A.; FRANCO, G. C. N.; GONÇALVES, R. B.; GROPPO, F. C. |
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Afiliação: |
Epagri |
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Título: |
Proteomic analysis of Porphyromonas gingivalis exposed to nicotine and cotinine. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
JOURNAL OF PERIODONTAL RESEARCH, Inglaterra, v. 1, n. 1, p. 1-10, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Cogo K, de Andrade A, Labate CA, Bergamaschi CC, Berto LA, Franco GCN, Gonçalves RB, Groppo FC. Proteomic analysis of Porphyromonas gingivalis exposed to nicotine and cotinine. J Periodont Res 2012; doi: 10.1111/j.1600-0765.2012.01494.x.©2012 John Wiley & Sons A/S Background and Objective: Smokers are more predisposed than nonsmokers to infection with Porphyromonas gingivalis, one of the most important pathogens involved in the onset and development of periodontitis. It has also been observed that tobacco, and tobacco derivatives such as nicotine and cotinine, can induce modifications to P. gingivalis virulence. However, the effect of the major compounds derived from cigarettes on expression of protein by P. gingivalis is poorly understood. Therefore, this study aimed to evaluate and compare the effects of nicotine and cotinine on the P. gingivalis proteomic profile. Material and Methods: Total proteins of P. gingivalis exposed to nicotine and cotinine were extracted and separated by two-dimensional electrophoresis. Proteins differentially expressed were successfully identified through liquid chromatography-mass spectrometry and primary sequence databases using MASCOT search engine, and gene ontology was carried out using DAVID tools. Results: Of the approximately 410 protein spots that were reproducibly detected on each gel, 23 were differentially expressed in at least one of the treatments. A particular increase was seen in proteins involved in metabolism, virulence and acquisition of peptides, protein synthesis and folding, transcription and oxidative stress. Few proteins showed significant decreases in expression; those that did are involved in cell envelope biosynthesis and proteolysis and also in metabolism. Conclusion: Our results characterized the changes in the proteome of P. gingivalis following exposure to nicotine and cotinine, suggesting that these substances may modulate, with minor changes, protein expression. The present study is, in part, a step toward understanding the potential smoke-pathogen interaction that may occur in smokers with periodontitis. MenosCogo K, de Andrade A, Labate CA, Bergamaschi CC, Berto LA, Franco GCN, Gonçalves RB, Groppo FC. Proteomic analysis of Porphyromonas gingivalis exposed to nicotine and cotinine. J Periodont Res 2012; doi: 10.1111/j.1600-0765.2012.01494.x.©2012 John Wiley & Sons A/S Background and Objective: Smokers are more predisposed than nonsmokers to infection with Porphyromonas gingivalis, one of the most important pathogens involved in the onset and development of periodontitis. It has also been observed that tobacco, and tobacco derivatives such as nicotine and cotinine, can induce modifications to P. gingivalis virulence. However, the effect of the major compounds derived from cigarettes on expression of protein by P. gingivalis is poorly understood. Therefore, this study aimed to evaluate and compare the effects of nicotine and cotinine on the P. gingivalis proteomic profile. Material and Methods: Total proteins of P. gingivalis exposed to nicotine and cotinine were extracted and separated by two-dimensional electrophoresis. Proteins differentially expressed were successfully identified through liquid chromatography-mass spectrometry and primary sequence databases using MASCOT search engine, and gene ontology was carried out using DAVID tools. Results: Of the approximately 410 protein spots that were reproducibly detected on each gel, 23 were differentially expressed in at least one of the treatments. A particular increase was seen in proteins involved in metabol... Mostrar Tudo |
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Palavras-Chave: |
Proteina; Proteoma. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02552naa a2200145 a 4500
001 1086117
005 2012-07-20
008 2012 bl uuuu u00u1 u #d
100 1 $aEpagri
245 $aProteomic analysis of Porphyromonas gingivalis exposed to nicotine and cotinine.
260 $c2012
520 $aCogo K, de Andrade A, Labate CA, Bergamaschi CC, Berto LA, Franco GCN, Gonçalves RB, Groppo FC. Proteomic analysis of Porphyromonas gingivalis exposed to nicotine and cotinine. J Periodont Res 2012; doi: 10.1111/j.1600-0765.2012.01494.x.©2012 John Wiley & Sons A/S Background and Objective: Smokers are more predisposed than nonsmokers to infection with Porphyromonas gingivalis, one of the most important pathogens involved in the onset and development of periodontitis. It has also been observed that tobacco, and tobacco derivatives such as nicotine and cotinine, can induce modifications to P. gingivalis virulence. However, the effect of the major compounds derived from cigarettes on expression of protein by P. gingivalis is poorly understood. Therefore, this study aimed to evaluate and compare the effects of nicotine and cotinine on the P. gingivalis proteomic profile. Material and Methods: Total proteins of P. gingivalis exposed to nicotine and cotinine were extracted and separated by two-dimensional electrophoresis. Proteins differentially expressed were successfully identified through liquid chromatography-mass spectrometry and primary sequence databases using MASCOT search engine, and gene ontology was carried out using DAVID tools. Results: Of the approximately 410 protein spots that were reproducibly detected on each gel, 23 were differentially expressed in at least one of the treatments. A particular increase was seen in proteins involved in metabolism, virulence and acquisition of peptides, protein synthesis and folding, transcription and oxidative stress. Few proteins showed significant decreases in expression; those that did are involved in cell envelope biosynthesis and proteolysis and also in metabolism. Conclusion: Our results characterized the changes in the proteome of P. gingivalis following exposure to nicotine and cotinine, suggesting that these substances may modulate, with minor changes, protein expression. The present study is, in part, a step toward understanding the potential smoke-pathogen interaction that may occur in smokers with periodontitis.
653 $aProteina
653 $aProteoma
773 $tJOURNAL OF PERIODONTAL RESEARCH, Inglaterra$gv. 1, n. 1, p. 1-10, 2012.
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