Data/hora: 03/06/2024 09:44:26

Biblioteca(s): | Epagri-Sede.
Data corrente: | 18/09/2019
Data da última atualização: | 18/09/2019
Autoria: | PEREIRA, A.; TCACENCO, F. A.; KLABUNDE, G. H. F.; ANDRADE, A.
Título: | Detecting acetyl-coenzyme a carboxylase resistance gene in rice (Oryza sativa L.).
Ano de publicação: | 2019
Fonte/Imprenta: | Molecular Biology Reports, New York, p. 1-6, 2019.
Idioma: | Inglês

Conteúdo:

Herbicides inhibiting acetyl-coenzyme A carboxylase (ACCase) are very efective in controlling grass weeds including
weedy-rice in paddy rice production systems. The ACCase inhibitor afects the enzyme by blocking fatty acid biosynthesis
resulting in plant death. The herbicide resistance in rice is conferred by a single point mutation with an amino acid substitution of the carboxyl transferase domain of the ACCase gene.
An assay based on the tetra-primer ARMS-PCR method was developed to detect the SNP G2027T that causes a tryptophan?cysteine substitution in the gene encoding chloroplastic
ACCase in rice. The protocol was tested in 453 rice samples from a segregant population for validation of the assay. This
technique can be exploited to monitor resistant lines in rice breeding programs to detect homozygous or heterozygous resistant
genotypes and homozygous susceptible genotypes. The presence of resistant ACCase allele(s) can be detected with rapidity,
simplicity, at low cost and can be used in any molecular biology laboratory with minimal equipment.

Palavras-Chave: | ACCase inhibitors; Allele-specifc PCR; Herbicide resistance; Rice breeding.
Categoria do assunto: | G Melhoramento Genético
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_43361.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (103885-1) - UPC - AP - DD -  -  -  - DIS