Data/hora: 03/06/2024 09:01:36

Biblioteca(s): | Epagri-Sede.
Data corrente: | 08/09/2015
Data da última atualização: | 08/09/2015
Autoria: | CUNHA, C. M. S.; HINZ, R. H.; PEREIRA, A.; TCACENCO, F. A.; PAULINO, E. C.; STADNIK, M. J.
Título: | A SCAR marker for identifying susceptibility to Fusarium oxysporum f. sp. cubense in banana.
Ano de publicação: | 2015
Fonte/Imprenta: | Scientia Horticulturae, British Columbia, v. 191, p. 108-112, 2015.
Idioma: | Inglês

Conteúdo:

Fusarium wilt (also known as Panama disease) caused by Fusarium oxysporum Schlechtend.
f. sp. cubense (E.F. Smith) W.C. Snyder & H.N. Hansen (Foc) is an endemic disease in all banana (Musa
spp. L.) producing areas. The use of resistant cultivars is the recommended method for the disease
control. The development of resistant or tolerant cultivars is expensive and time-consuming since the
results must be confirmed by many years of field evaluation. In this situation, DNA markers show a
great potential to improve the efficiency and precision of conventional plant breeding. Thus, the
objective of this work was to develop a SCAR marker able to discriminate between resistant and
susceptible genotypes to Foc infection. Using genomic DNA from resistant or susceptible genotypes
and 78 10-mer arbitrary primers, one RAPD band associated to susceptibility was selected and used to
generate the SCAR marker SuscPD-F/SuscPD-R. This marker was validated on 28 banana cultivars
which were resistant or susceptible to infection by Foc. The results indicated a high degree of
specificity of the marker, which was able to discriminate between the two contrasting groups
(resistant or susceptible). The discriminatory power of the new marker was 93%.

Palavras-Chave: | molecular marker; Panama disease.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_36452.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (99400-1) - UPC - AP - DD -  -  -  - DIS