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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
24/10/2019 |
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Data da última atualização: |
24/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BETTONI, J. C.; SOUZA, J. A.; VOLK, G. M.; COSTA, M. D.; SILVA, F. N.; KRETZSCHMAR, A. A. |
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Título: |
Eradication of latent viruses from apple cultivar 'Monalisa' shoot tips using droplet-vitrification cryotherapy. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Scientia Horticulturae, New York, n. 250, p. 12-18, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
In apple, virally transmitted diseases cause economic losses as a result of their direct effect on production and yield and by causing an increased susceptibility to other phytopathogenic agents. This study evaluated the effectiveness of a droplet-vitrification cryopreservation technique in eradicating latent viruses: Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV) and Apple stem pitting virus (ASPV) from in vitro axillary shoot tips excised from ?SC417 Monalisa? apple cultivar shoot tips. In vitro stock cultures, four weeks after subculture, infected with ASGV, ASPV and ACLSV were used as source material. Axillary shoot tips were excised from stock cultures, precultured in MS + 2 M glycerol + 0.8 M sucrose, and then exposed to plant vitrification solution 2 (PVS2) for between 0 and 80 min (six treatments) at 0 °C or room temperature prior to liquid nitrogen (LN) exposure. Shoot tips were then warmed and recovered on growth medium. The survival and regrowth of cryopreserved (+LN) and non-cryopreserved (-LN) 'Monalisa' shoot tips were evaluated and the efficiency of virus eradication was determined using RT-PCR after recovered plants were grown in a greenhouse for 6 months. With this protocol, the highest degree of regrowth (45%) were obtained after 20 to 40 min PVS2 exposure at room temperature. After 6 months of growth in a greenhouse, all of the plants regenerated after cryopreservation were free of ASPV, 95% were free of ACLSV, and 35% were free of ASGV. This promising cryotherapy procedure may facilitate the production of virus-free plants. MenosIn apple, virally transmitted diseases cause economic losses as a result of their direct effect on production and yield and by causing an increased susceptibility to other phytopathogenic agents. This study evaluated the effectiveness of a droplet-vitrification cryopreservation technique in eradicating latent viruses: Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV) and Apple stem pitting virus (ASPV) from in vitro axillary shoot tips excised from ?SC417 Monalisa? apple cultivar shoot tips. In vitro stock cultures, four weeks after subculture, infected with ASGV, ASPV and ACLSV were used as source material. Axillary shoot tips were excised from stock cultures, precultured in MS + 2 M glycerol + 0.8 M sucrose, and then exposed to plant vitrification solution 2 (PVS2) for between 0 and 80 min (six treatments) at 0 °C or room temperature prior to liquid nitrogen (LN) exposure. Shoot tips were then warmed and recovered on growth medium. The survival and regrowth of cryopreserved (+LN) and non-cryopreserved (-LN) 'Monalisa' shoot tips were evaluated and the efficiency of virus eradication was determined using RT-PCR after recovered plants were grown in a greenhouse for 6 months. With this protocol, the highest degree of regrowth (45%) were obtained after 20 to 40 min PVS2 exposure at room temperature. After 6 months of growth in a greenhouse, all of the plants regenerated after cryopreservation were free of ASPV, ... Mostrar Tudo |
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Palavras-Chave: |
Apple chlorotic leaf spot virus; Apple stem pitting virus; Cryopreservation; High-health plants; Malu; s Apple stem grooving virus. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02428naa a2200253 a 4500
001 1129000
005 2019-10-24
008 2019 bl uuuu u00u1 u #d
100 1 $aBETTONI, J. C.
245 $aEradication of latent viruses from apple cultivar 'Monalisa' shoot tips using droplet-vitrification cryotherapy.$h[electronic resource]
260 $c2019
520 $aIn apple, virally transmitted diseases cause economic losses as a result of their direct effect on production and yield and by causing an increased susceptibility to other phytopathogenic agents. This study evaluated the effectiveness of a droplet-vitrification cryopreservation technique in eradicating latent viruses: Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV) and Apple stem pitting virus (ASPV) from in vitro axillary shoot tips excised from ?SC417 Monalisa? apple cultivar shoot tips. In vitro stock cultures, four weeks after subculture, infected with ASGV, ASPV and ACLSV were used as source material. Axillary shoot tips were excised from stock cultures, precultured in MS + 2 M glycerol + 0.8 M sucrose, and then exposed to plant vitrification solution 2 (PVS2) for between 0 and 80 min (six treatments) at 0 °C or room temperature prior to liquid nitrogen (LN) exposure. Shoot tips were then warmed and recovered on growth medium. The survival and regrowth of cryopreserved (+LN) and non-cryopreserved (-LN) 'Monalisa' shoot tips were evaluated and the efficiency of virus eradication was determined using RT-PCR after recovered plants were grown in a greenhouse for 6 months. With this protocol, the highest degree of regrowth (45%) were obtained after 20 to 40 min PVS2 exposure at room temperature. After 6 months of growth in a greenhouse, all of the plants regenerated after cryopreservation were free of ASPV, 95% were free of ACLSV, and 35% were free of ASGV. This promising cryotherapy procedure may facilitate the production of virus-free plants.
653 $aApple chlorotic leaf spot virus
653 $aApple stem pitting virus
653 $aCryopreservation
653 $aHigh-health plants
653 $aMalu
653 $as Apple stem grooving virus
700 1 $aSOUZA, J. A.
700 1 $aVOLK, G. M.
700 1 $aCOSTA, M. D.
700 1 $aSILVA, F. N.
700 1 $aKRETZSCHMAR, A. A.
773 $tScientia Horticulturae, New York$gn. 250, p. 12-18, 2019.
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Registro original: |
Epagri-Sede (Epagri-Sede) |
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| Registros recuperados : 15 | |
| 2. |  | ARAUJO, L.; MEDEIROS, H. A.; PASA, M. S.; SILVA, F. N. Doenças da macieira e da pereira. Informe Agropecuária, Belo Horizonte, MG, v. 37, n. 291, p. 61-74, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - C |
| Biblioteca(s): Epagri-Sede. |
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| 3. | | BETTONI, J. C.; COSTA, M. D.; SOUZA, J. A.; VOLK, G. M.; NICKEL, O.; SILVA, F. N.; KRETZSCHMAR, A. A. Cryotherapy by encapsulation-dehydration is effective for in vitro eradication of latent viruses from 'Marubakaido' apple rootstock. Journal of Biotechnology, Amsterdã, v. 269, p. 1-7, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
| Biblioteca(s): Epagri-Sede. |
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| 4. | | DALLA COSTA, M.; RECH, T. D.; KVITSCHAL, M. V.; SILVA, F. N.; FAJARDO, T. V. M.; PASSOS, J. F. M. Regeneração de ápices meristemáticos de culturas in vitro de macieira após tratamento com compostos antivirais. In: SIMPÓSIO DE FRUTICULTURA DA REGIÃO SUL, 3., 2022, Online. Resumos... Chapecó: Epagri, 2022.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 5. | | SOUZA, J. A.; BOGO, A.; BETTONI, J. C.; COSTA, M. D.; SILVA, F. N.; CASA, R. T.; RUFATO, L. Droplet-vitrification cryotherapy for eradication of apple stem grooving virus and apple stem pitting virus from ?Marubakaido? apple rootstock. Tropical Plant Pathology, Brasília, v. 45, n. , p. 148-152, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Epagri-Sede. |
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| 6. | | BETTONI, J. C.; SOUZA, J. A.; VOLK, G. M.; COSTA, M. D.; SILVA, F. N.; KRETZSCHMAR, A. A. Eradication of latent viruses from apple cultivar 'Monalisa' shoot tips using droplet-vitrification cryotherapy. Scientia Horticulturae, New York, n. 250, p. 12-18, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
| Biblioteca(s): Epagri-Sede. |
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| 7. | | SILVA, M. C. C. R.; FERREIRA, J.; GORAYEB, E. S.; SAVARIS, D. M.; BORGES, V.; SILVA, F. N.; RIBEIRO, L. P. Infectivity of corn leafhopper, Dalbulus maidis (Hemiptera: Cicadellidae), with Maize bushy stunt phytoplasma and Spiroplasma kunkelii in Santa Catarina State, Brazil. In: WORKSHOP BRASILEIRO DE EPIDEMIOLOGIA DE DOENÇAS DE PLANTAS, 6., 2022, Chapecó. Resumos... Brasília: Sociedade Brasileira de Fitopatologia, 2022. p. 42| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 8. | | Canale, M. C.; ANDRADE, M. V. S.; ZINGER, L. K. C. R.; GORAYEB, E. S.; RIBEIRO, L. P.; SILVA, F. N. COMPLEXO DE ENFEZAMENTOS EM MILHO: ASPECTOS FITOPATOLÓGICOS E MONITORAMENTO DA CIGARRINHA VETORA EM SANTA CATARINA. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília. Resumos... Brasília: Sociedade Brasileira de Fitopatologia, 2023. p. 746| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 9. | | NASCIMENTO, S. C.; ALBUQUERQUE, M. R. M.; GORAYEB, E. S.; SARTORI, F.; SCHEUERMANN, K. K.; MELLO, R. N.; MENDES, G. C.; SILVA, F. N. Quantificação de Rice Stripe Necrosis Virus (RSNV) e do seu vetor Polymyxa graminis em diferentes genótipos de arroz. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília. Resumos... Brasília: Sociedade Brasileira de Fitopatologia, 2023. p. 702| Tipo: Resumo em Anais de Congresso |
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| 10. | | CANALE, M. C.; ANDRADE, M. V. S.; GORAYEB, E. S.; ZINGER, L. K. C. R.; CÓL, M. C.; PONTE, E. M. D.; SILVA, F. N. Natural infectivity of the corn leafhopper, Dalbulus maidis, with the Corn Stunting Complex pathogens in Santa Catarina, Brazil. In: INTERNATIONAL EPIDEMIOLOGY WORKSHOP, 13., 2024, Foz do Iguaçú, PR. Abstracts... Viçosa, MG: UFV, 2024. p. 40| Tipo: Resumo em Anais de Congresso |
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| 11. | | SAVARIS, D. M.; RIBEIRO, L. P.; FERREIRA, J.; SILVA, M. C. C. R.; NESI, C. N.; GORAYEB, E.; SILVA, F. N. Incidência de Dalbulus maidis (Hemiptera: Cicadellidae) em lavouras de milho de Santa Catarina, Brasil: efeito da posição da armadilha no talhão e da vegetação de entorno. In: ENCONTRO SUL-BRASILEIRO DE FITOSSANIDADE, 1., 2022, Chapecó, SC. Anais... Chapecó, SC: Faem Faculdades, 2022.| Tipo: Resumo em Anais de Congresso |
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| 12. | | PIVA, C. A. G.; BOGO, A.; GOMES, B.; MILCHESKI, V.; KLABUNDE, G. H. F.; NODARI, R. O.; SILVA, F. N.; WELTER, L. J. Pestalotiopsis spp. causing grapevine leaf spot and postharvest berry rot in southern Brazil. ACTA HORTICULTURAE, Leuven, Bélgica, n. 1280, p. 209-216, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
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| 13. | | SILVA, F. N.; FERREIRA, J.; GORAYEB, E. S.; SOUZA, V. B.; SAVARIS, D. M.; SILVA, M. C. C. R.; RIBEIRO, L. P.; ALBUQUERQUE, M. R. M.; CAMARGO, M. P.; STOCK, V. Dalbulus maidis and corn stunt pathogens monitoring program in Santa Catarina, Brazil: Scientific advancements and challenges. In: WORKSHOP BRASILEIRO DE EPIDEMIOLOGIA DE DOENÇAS DE PLANTAS, 6., 2022, Chapecó. Resumos... Brasília: Sociedade Brasileira de Fitopatologia, 2022. p. 14| Tipo: Resumo em Anais de Congresso |
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| 14. | | GORAYEB, E. S.; ALBUQUERQUE, M. R. M.; FERREIRA, J.; NASCIMENTO, S. C.; SILVA, T. S.; SAVARIS, D. M.; RIBEIRO, L. P.; SILVA, M. C. C. R.; SILVA, F. N. Nucleic acid extraction and multiplex analysis for simultaneous detection of the corn stunt complex pathogens in plant and insect tissues. Tropical Plant Pathology, Viçosa, MG, 2022.| Tipo: Artigo em Periódico Indexado |
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| 15. | | GORAYEB, E. S.; ALBUQUERQUE, M. R. M.; FERREIRA, J.; NASCIMENTO, S. C.; SILVA, T. S.; SAVARIS, D. M.; RIBEIRO, L. P.; SILVA, M. C. C. R.; SILVA, F. N. Nucleic acid extraction and multiplex polymerase chain reaction analysis for the detection of maize stunt causal agents in plants and insect tissues. In: WORKSHOP BRASILEIRO DE EPIDEMIOLOGIA DE DOENÇAS DE PLANTAS, 6., 2022, Chapecó. Resumos... Brasília: Sociedade Brasileira de Fitopatologia, 2022. p. 37| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| Registros recuperados : 15 | |
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