Data/hora: 26/06/2024 11:42:22

Biblioteca(s): | Epagri-Sede.
Data corrente: | 24/10/2019
Data da última atualização: | 24/10/2019
Autoria: | BETTONI, J. C.; SOUZA, J. A.; VOLK, G. M.; COSTA, M. D.; SILVA, F. N.; KRETZSCHMAR, A. A.
Título: | Eradication of latent viruses from apple cultivar 'Monalisa' shoot tips using droplet-vitrification cryotherapy.
Ano de publicação: | 2019
Fonte/Imprenta: | Scientia Horticulturae, New York, n. 250, p. 12-18, 2019.
Idioma: | Inglês
Conteúdo: | In apple, virally transmitted diseases cause economic losses as a result of their direct effect on production and yield and by causing an increased susceptibility to other phytopathogenic agents. This study evaluated the effectiveness of a droplet-vitrification cryopreservation technique in eradicating latent viruses: Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV) and Apple stem pitting virus (ASPV) from in vitro axillary shoot tips excised from ?SC417 Monalisa? apple cultivar shoot tips. In vitro stock cultures, four weeks after subculture, infected with ASGV, ASPV and ACLSV were used as source material. Axillary shoot tips were excised from stock cultures, precultured in MS&#8201;+&#8201;2&#8201;M glycerol + 0.8&#8201;M sucrose, and then exposed to plant vitrification solution 2 (PVS2) for between 0 and 80&#8201;min (six treatments) at 0&#8201;°C or room temperature prior to liquid nitrogen (LN) exposure. Shoot tips were then warmed and recovered on growth medium. The survival and regrowth of cryopreserved (+LN) and non-cryopreserved (-LN) 'Monalisa' shoot tips were evaluated and the efficiency of virus eradication was determined using RT-PCR after recovered plants were grown in a greenhouse for 6 months. With this protocol, the highest degree of regrowth (45%) were obtained after 20 to 40&#8201;min PVS2 exposure at room temperature. After 6 months of growth in a greenhouse, all of the plants regenerated after cryopreservation were free of ASPV, 95% were free of ACLSV, and 35% were free of ASGV. This promising cryotherapy procedure may facilitate the production of virus-free plants.
Palavras-Chave: | Apple chlorotic leaf spot virus; Apple stem pitting virus; Cryopreservation; High-health plants; Malu; s Apple stem grooving virus.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_43630.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (104001-1) - UPC - AP - DD -  -  -  - DIS