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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
28/02/2013 |
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Data da última atualização: |
28/02/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TOMAZELLI, I. B.; TOMAZELLI JÚNIOR, O.; FREITAS, J. B.; FABBIA, L. M.; FILIPINI, T. A.; SILVA, C. M.; BEDIN, J. M.; DUARTE, D. A. M.; SANTOS, A.; BACCARIN, A.; HIGA, L. R. G.; YANO, D. M. Y.; KILLNER, M.; FREZZA, A. L. C.; ABECIA, E. C. G.; TRONCO, V. M.; J¨²NIOR, W. B. |
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Afiliação: |
Epagri |
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Título: |
Comparison of the BAX System PCR Method to Brazil's Official Method for the Detection of Salmonella in Food, Water, and Environmental Samples. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Journal of Food Protection, v. 71, n. 12, p. 2442-2447, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
A two-stage study compared the BAX system PCR method with the reference culture method used by the Brazilian Ministry of Agriculture and Food Supply for the detection of Salmonella in food, water, and environmental samples. In stage 1, fish matrix samples (n = 258) were spiked at several levels with Salmonella and a combination of Salmonella and non-Salmonella competitive organisms. Replicates were analyzed by the BAX system PCR method and the reference method with comparable results (sensitivity ¡Ý 97.5%, specificity ¡Ý 83.3%) from both methods at the limit of detection. In stage 2, a total of 1,988 samples with 70 product types were analyzed with both methods. Five laboratories were involved in this study, and the samples used were from routine analyses. The BAX system PCR method was shown to be comparable to the reference method, with a limit of detection of 1.0 to 2.0 CFU/25 g of sample. Analysis of the results obtained in stage 2 and in the combination of stages 1 and 2 for the BAX system showed the following performance: sensitivity ¡Ý 99.0%, specificity ¡Ý 97.2%, false-negative rate ¡Ü 1.1%, and false-positive rate ¡Ü 2.8%. Therefore, the BAX system appears to be equivalent to the reference method, with ¡Ý97.3% agreement. |
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Palavras-Chave: |
Brazil; Detection of Salmonella; M¨¦todo; PCR. |
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Categoria do assunto: |
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Marc: |
LEADER 02298naa a2200361 a 4500
001 1088405
005 2013-02-28
008 2008 bl uuuu u00u1 u #d
100 1 $aTOMAZELLI, I. B.
245 $aComparison of the BAX System PCR Method to Brazil's Official Method for the Detection of Salmonella in Food, Water, and Environmental Samples.
260 $c2008
520 $aA two-stage study compared the BAX system PCR method with the reference culture method used by the Brazilian Ministry of Agriculture and Food Supply for the detection of Salmonella in food, water, and environmental samples. In stage 1, fish matrix samples (n = 258) were spiked at several levels with Salmonella and a combination of Salmonella and non-Salmonella competitive organisms. Replicates were analyzed by the BAX system PCR method and the reference method with comparable results (sensitivity ¡Ý 97.5%, specificity ¡Ý 83.3%) from both methods at the limit of detection. In stage 2, a total of 1,988 samples with 70 product types were analyzed with both methods. Five laboratories were involved in this study, and the samples used were from routine analyses. The BAX system PCR method was shown to be comparable to the reference method, with a limit of detection of 1.0 to 2.0 CFU/25 g of sample. Analysis of the results obtained in stage 2 and in the combination of stages 1 and 2 for the BAX system showed the following performance: sensitivity ¡Ý 99.0%, specificity ¡Ý 97.2%, false-negative rate ¡Ü 1.1%, and false-positive rate ¡Ü 2.8%. Therefore, the BAX system appears to be equivalent to the reference method, with ¡Ý97.3% agreement.
653 $aBrazil
653 $aDetection of Salmonella
653 $aM¨¦todo
653 $aPCR
700 1 $aTOMAZELLI JÚNIOR, O.
700 1 $aFREITAS, J. B.
700 1 $aFABBIA, L. M.
700 1 $aFILIPINI, T. A.
700 1 $aSILVA, C. M.
700 1 $aBEDIN, J. M.
700 1 $aDUARTE, D. A. M.
700 1 $aSANTOS, A.
700 1 $aBACCARIN, A.
700 1 $aHIGA, L. R. G.
700 1 $aYANO, D. M. Y.
700 1 $aKILLNER, M.
700 1 $aFREZZA, A. L. C.
700 1 $aABECIA, E. C. G.
700 1 $aTRONCO, V. M.
700 1 $aJ¨²NIOR, W. B.
773 $tJournal of Food Protection$gv. 71, n. 12, p. 2442-2447, 2008.
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