Data/hora: 08/06/2024 13:32:03

Biblioteca(s): | Epagri-Sede.
Data corrente: | 12/08/2020
Data da última atualização: | 12/08/2020
Autoria: | VENTURA, T. F. B.; BRUZINGA, C. P.; SANTOS, A. A.; SIMIONI, C.; HAYASHI, L.
Título: | Addition of carbon dioxide, followed by irradiance increase, as optimization strategy for the cultivation of the red seaweed Kappaphycus alvarezii.
Ano de publicação: | 2020
Fonte/Imprenta: | Journal of Applied Phycology, Germany, p. 1-14, 2020.
Idioma: | Inglês
Conteúdo: | This work aimed to verify the effects of adding CO2 and irradiance to Kappaphycus alvarezii cultivation. Thalli were cultured in vitro for 2 weeks under low irradiance (50 &#956;mol photons m&#8722;2 s &#8722;1) with the addition of CO2 according to the following concentrations: 142× 103 ppm (1CO2, 0.1 L min&#8722;1), 285 × 103 ppm (2CO2, 0.2 L min&#8722;1) and 428 × 103 ppm (3CO2, 0.3 L min&#8722;1),followed by culture under higher irradiance (200 &#956;mol photons m&#8722;2 s&#8722;1) for 2 more weeks. Control had no CO2 addition. Afterwards, growth rate, chlorophyll a and carotenoid content were quantified. Samples were submitted to light microscopy and transmission electron microscopy. Growth rates of different treatments or control between experimental periods showed no significant differences, except for 2CO2 treatment at the end of each period. Kappaphycus alvarezii cultivated with CO2 addition showed cell wall thickening and increasing quantity of starch granules, chlorophyll a and carotenoids compared with control. Exposed to high irradiance, control samples showed signs of stress, such as changes in chloroplast, starch granules quantity decrease and total chlorophyll a and carotenoids increase. Samples cultivated with CO2 showed an increase in the quantity and size of the starch granules and an increase in the number of organelles (mitochondria) related to energy generation and cell construction (Golgi complex). Such alterations suggest cellular response after CO2 addition, such as the formation of starch reserves and cell wall thickening, which could make plants more tolerant to environmental stress during transport from indoor condition to sea farms.
Palavras-Chave: | Aquaculture; Electron microscopy.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_46329.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
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