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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
05/03/2013 |
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Data da última atualização: |
05/03/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VISTUBA, J. P.; PIOVEZAN, M.; PIZZOLATTI, M. G.; REBELO, A. M.; AZEVEDO, M. S.; VITALI, L.; COSTA, A. C. O.; MICKE, G. A. |
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Afiliação: |
Epagri |
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Título: |
Increasing the instrumental throughput of gas chromatography method using multiple injections in a single experimental run Application in determination of friedelan3ol and friedelin in Maytenus ilicifolia. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Chromatography A, USA, n. 1274, p. 159-164, 2013. |
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Idioma: |
Português |
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Conteúdo: |
The aim of this study was to develop a separation method using multiple injections in a single experimen-tal run (MISER) employing gas chromatography with flame ionization detection (GC-FID) for the first time, without instrument modification, to increase the instrumental throughput. The method was applied to the determination of the triterpenes friedelan-3-ol and friedelin in a plant extract obtained from Maytenus ilicifolia leaves. The column used in the chromatography system was a ZB-50 (30 m × 0.25 mm × 0.15 m) with 50% phenyl?50% methyl-polysiloxane. The samples (1.0 L) were injected using the split mode (1:90), with the injector at 280◦C and FID detector at 320◦C. The column temperature was programmed to isothermal mode at 300◦C. Helium was used as the carrier gas with a constant flow of 1.5 mL min−1. It was possible to perform three injections in the same run using the MISER method. An increase in the instrumental throughput by a factor of around 2.6 was observed when the use of multiple injections was compared with the single injection method. This method showed good linearity for both analytes with R2> 0.99 and the values for the limit of detection were 0.24 mg L−1 and 0.44 mg L−1, for friedelan-3-ol and friedelin, respectively. Furthermore, the values for the limits of quantification were 0.79 mg L −1 for friedelan-3-ol and 1.16 mg L −1 for friedelin. The proposed method has potential for other GC applica-tions, allowing an increase in the instrumental throughput of the methods by applying this separation technique. MenosThe aim of this study was to develop a separation method using multiple injections in a single experimen-tal run (MISER) employing gas chromatography with flame ionization detection (GC-FID) for the first time, without instrument modification, to increase the instrumental throughput. The method was applied to the determination of the triterpenes friedelan-3-ol and friedelin in a plant extract obtained from Maytenus ilicifolia leaves. The column used in the chromatography system was a ZB-50 (30 m × 0.25 mm × 0.15 m) with 50% phenyl?50% methyl-polysiloxane. The samples (1.0 L) were injected using the split mode (1:90), with the injector at 280◦C and FID detector at 320◦C. The column temperature was programmed to isothermal mode at 300◦C. Helium was used as the carrier gas with a constant flow of 1.5 mL min−1. It was possible to perform three injections in the same run using the MISER method. An increase in the instrumental throughput by a factor of around 2.6 was observed when the use of multiple injections was compared with the single injection method. This method showed good linearity for both analytes with R2> 0.99 and the values for the limit of detection were 0.24 mg L−1 and 0.44 mg L−1, for friedelan-3-ol and friedelin, respectively. Furthermore, the values for the limits of quantification were 0.79 mg L −1 for friedelan-3-ol and 1.16 mg L −1 for friedelin. The proposed method has potential for other GC applica-tions, allow... Mostrar Tudo |
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Palavras-Chave: |
MISER. |
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Categoria do assunto: |
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Marc: |
LEADER 02327naa a2200217 a 4500
001 1088446
005 2013-03-05
008 2013 bl uuuu u00u1 u #d
100 1 $aVISTUBA, J. P.
245 $aIncreasing the instrumental throughput of gas chromatography method using multiple injections in a single experimental run Application in determination of friedelan3ol and friedelin in Maytenus ilicifolia.
260 $c2013
520 $aThe aim of this study was to develop a separation method using multiple injections in a single experimen-tal run (MISER) employing gas chromatography with flame ionization detection (GC-FID) for the first time, without instrument modification, to increase the instrumental throughput. The method was applied to the determination of the triterpenes friedelan-3-ol and friedelin in a plant extract obtained from Maytenus ilicifolia leaves. The column used in the chromatography system was a ZB-50 (30 m × 0.25 mm × 0.15 m) with 50% phenyl?50% methyl-polysiloxane. The samples (1.0 L) were injected using the split mode (1:90), with the injector at 280◦C and FID detector at 320◦C. The column temperature was programmed to isothermal mode at 300◦C. Helium was used as the carrier gas with a constant flow of 1.5 mL min−1. It was possible to perform three injections in the same run using the MISER method. An increase in the instrumental throughput by a factor of around 2.6 was observed when the use of multiple injections was compared with the single injection method. This method showed good linearity for both analytes with R2> 0.99 and the values for the limit of detection were 0.24 mg L−1 and 0.44 mg L−1, for friedelan-3-ol and friedelin, respectively. Furthermore, the values for the limits of quantification were 0.79 mg L −1 for friedelan-3-ol and 1.16 mg L −1 for friedelin. The proposed method has potential for other GC applica-tions, allowing an increase in the instrumental throughput of the methods by applying this separation technique.
653 $aMISER
700 1 $aPIOVEZAN, M.
700 1 $aPIZZOLATTI, M. G.
700 1 $aREBELO, A. M.
700 1 $aAZEVEDO, M. S.
700 1 $aVITALI, L.
700 1 $aCOSTA, A. C. O.
700 1 $aMICKE, G. A.
773 $tJournal of Chromatography A, USA$gn. 1274, p. 159-164, 2013.
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| 2. |  | VISTUBA, J. P.; PIOVEZAN, M.; PIZZOLATTI, M. G.; REBELO, A. M.; AZEVEDO, M. S.; VITALI, L.; COSTA, A. C. O.; MICKE, G. A. Increasing the instrumental throughput of gas chromatography method using multiple injections in a single experimental run Application in determination of friedelan3ol and friedelin in Maytenus ilicifolia. Journal of Chromatography A, USA, n. 1274, p. 159-164, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - -- |
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