Data/hora: 16/06/2024 07:58:10

Biblioteca(s): | Epagri-Sede.
Data corrente: | 05/03/2013
Data da última atualização: | 05/03/2013
Autoria: | VISTUBA, J. P.; PIOVEZAN, M.; PIZZOLATTI, M. G.; REBELO, A. M.; AZEVEDO, M. S.; VITALI, L.; COSTA, A. C. O.; MICKE, G. A.
Afiliação: | Epagri
Título: | Increasing the instrumental throughput of gas chromatography method using multiple injections in a single experimental run Application in determination of friedelan3ol and friedelin in Maytenus ilicifolia.
Ano de publicação: | 2013
Fonte/Imprenta: | Journal of Chromatography A, USA,  n. 1274, p. 159-164, 2013.
Idioma: | Português
Conteúdo: | The aim of this study was to develop a separation method using multiple injections in a single experimen-tal run (MISER) employing gas chromatography with flame ionization detection (GC-FID) for the first time, without instrument modification, to increase the instrumental throughput. The method was applied to the determination of the triterpenes friedelan-3-ol and friedelin in a plant extract obtained from Maytenus ilicifolia leaves. The column used in the chromatography system was a ZB-50 (30 m × 0.25 mm × 0.15 m) with 50% phenyl?50% methyl-polysiloxane. The samples (1.0 L) were injected using the split mode (1:90), with the injector at 280&#9702;C and FID detector at 320&#9702;C. The column temperature was programmed to isothermal mode at 300&#9702;C. Helium was used as the carrier gas with a constant flow of 1.5 mL min&#8722;1. It was possible to perform three injections in the same run using the MISER method. An increase in the instrumental throughput by a factor of around 2.6 was observed when the use of multiple injections was compared with the single injection method. This method showed good linearity for both analytes with R2> 0.99 and the values for the limit of detection were 0.24 mg L&#8722;1 and 0.44 mg L&#8722;1, for friedelan-3-ol and friedelin, respectively. Furthermore, the values for the limits of quantification were 0.79 mg L &#8722;1 for friedelan-3-ol and 1.16 mg L &#8722;1 for friedelin. The proposed method has potential for other GC applica-tions, allowing an increase in the instrumental throughput of the methods by applying this separation technique.
Palavras-Chave: | MISER.
Categoria do assunto: | --
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_26870.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
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