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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
03/06/2020 |
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Data da última atualização: |
03/06/2020 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
ROSSAROLLA, M. D.; TOMAZETTI, T. C.; VIEIRA, L. N.; GUERRA, M. P.; KLABUNDE, G. H. F.; SCHERER, R. F.; PESCADOR, R.; NODARI, R. O. |
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Título: |
Identification and characterization of SSR markers of Guadua chacoensis (Rojas) Londoño & P.M. Peterson and transferability to other bamboo species. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
3 Biotech, Suíça, v. 10, p. 1-9, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to develop simple sequence repeat (SSR) markers for genetic studies on G. chacoensis, as well as to
evaluate their transferability to other bamboo species. Genomic DNA was isolated from G. chacoensis and its partial sequencing
was used to find SSR loci. The obtained sequencing data were de novo assembled using the software CLC Genomics
Workbench ® 8.0v. The SSR loci primers were identified and designed with the software SSRLocator. The selected markers
were validated using 56 plants sampled in seven populations from southern Brazil. The markers with potential polymorphism
were selected and fluorescently labeled for characterization by capillary electrophoresis. In total, 92 SSR loci were
found in G. chacoensis contigs. Suitable primers were designed for 70 SSR loci, and the remaining 22 SSR loci did not have
sequences for primer development. Out of 35 selected SSR markers, after PCR optimization, 10 with high polymorphism
potential were characterized. These loci can be used in genetic analyses of G. chacoensis and all of them were successfully
transferred to other bamboo species. Non-polymorphic loci require further tests with additional plants, from different populations,
to identify possibilities of their use. |
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Palavras-Chave: |
Genetic diversity; Genetic population; Molecular markers; Polymorphism; Primer design; SSR. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02098naa a2200277 a 4500
001 1129683
005 2020-06-03
008 2020 bl uuuu u00u1 u #d
100 1 $aROSSAROLLA, M. D.
245 $aIdentification and characterization of SSR markers of Guadua chacoensis (Rojas) Londoño & P.M. Peterson and transferability to other bamboo species.$h[electronic resource]
260 $c2020
520 $aThe aim of this study was to develop simple sequence repeat (SSR) markers for genetic studies on G. chacoensis, as well as to evaluate their transferability to other bamboo species. Genomic DNA was isolated from G. chacoensis and its partial sequencing was used to find SSR loci. The obtained sequencing data were de novo assembled using the software CLC Genomics Workbench ® 8.0v. The SSR loci primers were identified and designed with the software SSRLocator. The selected markers were validated using 56 plants sampled in seven populations from southern Brazil. The markers with potential polymorphism were selected and fluorescently labeled for characterization by capillary electrophoresis. In total, 92 SSR loci were found in G. chacoensis contigs. Suitable primers were designed for 70 SSR loci, and the remaining 22 SSR loci did not have sequences for primer development. Out of 35 selected SSR markers, after PCR optimization, 10 with high polymorphism potential were characterized. These loci can be used in genetic analyses of G. chacoensis and all of them were successfully transferred to other bamboo species. Non-polymorphic loci require further tests with additional plants, from different populations, to identify possibilities of their use.
653 $aGenetic diversity
653 $aGenetic population
653 $aMolecular markers
653 $aPolymorphism
653 $aPrimer design
653 $aSSR
700 1 $aTOMAZETTI, T. C.
700 1 $aVIEIRA, L. N.
700 1 $aGUERRA, M. P.
700 1 $aKLABUNDE, G. H. F.
700 1 $aSCHERER, R. F.
700 1 $aPESCADOR, R.
700 1 $aNODARI, R. O.
773 $t3 Biotech, Suíça$gv. 10, p. 1-9, 2020.
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Epagri-Sede (Epagri-Sede) |
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| 21. |  | BRIGHENTI, L. H. M.; SAUTCHUCK, S.; MALOHLAVA, I. T. C.; VOTRE, T. C. G.; FELINI, V. S.; PINTO, F. A. M. F.; BRIGHENTI, A. F.; PESCADOR, R. Vegetative propagation of hops (Humulus lupulus), effect of biostimulants, size and position of cuttings. Revista de Ciências Agroveterinárias, Florianópolis, v. 22, n. 4, p. 554-562, 2023.| Tipo: Artigo em Periódico Indexado |
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